B2UR52 (ILVD_AKKM8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Amuc_1111

Organism

Akkermansia muciniphila (strain ATCC BAA-835) [Complete proteome] [HAMAP]

Taxonomic identifier

349741 [NCBI]

Taxonomic lineage

Bacteria › Verrucomicrobia › Verrucomicrobiae › Verrucomicrobiales › Verrucomicrobiaceae › Akkermansia

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089365
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B2UR52 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 6BC40992FFC7A2A6	FASTA	558	59,476
	10 20 30 40 50 60 MRSDRVKAGF ERAPHRSLMR ATGMTDEDLS RPFIAICNSF NEVIPGHVHL NRVAALIKEE 70 80 90 100 110 120 VRKAGGTPVE FNLPGVCDGI AMGHGGMKFS LASRELIADS VETMLSAHAF DAMICIPNCD 130 140 150 160 170 180 KIVPGMIMGA LRCNIPTIFC SGGPMAAGMA EDGTVLDLNS VFEAVARFKA GKINEEELHS 190 200 210 220 230 240 LECRACPGAG SCSGMFTANS MNCLSEVIGL ALPGNGSLLA TSEERKEFWK QTARRAVEMA 250 260 270 280 290 300 KADGPLPRDI VTRDAIDNAF TIDMAMGGSS NTVLHTLAIA REAGVEYDLQ RINDISRRTP 310 320 330 340 350 360 NICKVAPSSR FHMQDVLRAG GVSAIIHEIA RIPGALHLDA MTVSGKTLGE TVEGCGIADE 370 380 390 400 410 420 TVIHPLENAY SRDGGLAILF GNLAEEGAVV KKAGVHPNMM SFRGPAVIFE SQEEACEGIL 430 440 450 460 470 480 AGKVKSGDVV VIRNEGPKGG PGMQEMLAPT SYIMGQGLGA EVALITDGRF SGATHGACIG 490 500 510 520 530 540 HISPEAAEGG LIGLLRNGDI IEYSIPDRTL NVCLSEEEIA RRRADWKPTY NRVSSSWLSR 550 YRQLATNASK GAVLRRGE

References

[1]

"Complete sequence of Akkermansia muciniphila ATCC BAA-835." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N. , Smidt H., Derrien M., Plugge C.M., Zoetendal E.G., de Vos W.M., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-835.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001071 Genomic DNA. Translation: ACD04937.1.
RefSeq	YP_001877718.1. NC_010655.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2UR52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6273966.
GenomeReviews	Gene locus Amuc_1111 in contig CP001071_GR.
KEGG	amu:Amuc_1111.
PATRIC	20835028. VBIAkkMuc16855_1258.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_AKKM8

Accession

Primary (citable) accession number: B2UR52

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program