B2UNU8 (GLAB_AKKM8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-galactosidase B
EC=3.2.1.n1
EC=3.2.1.n2

Alternative name(s):
Exo-alpha-galactosidase B
EC=3.2.1.22

Gene names

Name:	glaB
Ordered Locus Names:	Amuc_0480

Organism

Akkermansia muciniphila (strain ATCC BAA-835) [Complete proteome] [HAMAP]

Taxonomic identifier

349741 [NCBI]

Taxonomic lineage

Bacteria › Verrucomicrobia › Verrucomicrobiae › Verrucomicrobiales › Verrucomicrobiaceae › Akkermansia

Protein attributes

Sequence length	794 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alpha-galactosidase. Removes both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Hydrolysis of terminal, non-reducing linear (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. B subfamily. Contains 5 PbH1 repeats.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 57	57	Potential
	Chain	58 – 794	737	Alpha-1,3-galactosidase B		PRO_0000348475
Regions
	Repeat	468 – 499	32	PbH1 1
	Repeat	609 – 631	23	PbH1 2
	Repeat	632 – 654	23	PbH1 3
	Repeat	665 – 686	22	PbH1 4
	Repeat	707 – 728	22	PbH1 5

Sequences

	Sequence		Length	Mass (Da)
	B2UNU8 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: CDEF0FFFD10D164A	FASTA	794	87,763
	10 20 30 40 50 60 MEGNLSFSLM EASGRSIFFL IEGIREQSIK NMFSRMFSWS FVVAACLAGL FPAQSQGEEK 70 80 90 100 110 120 AAQSGTIAVK VPASSLLMTR QETGETRLDR SFSNAGLSIG GKKYATGIGT HATSMIPLPV 130 140 150 160 170 180 PENPKVLRLE GACGIDDGAD GDGSVEFRVM SGSEVLWSSG VMRRGMAAKK FSIPVAENGI 190 200 210 220 230 240 RHLYLMADRV DNNSYDHADW VDLAWKTTGS GQGMKGAVVN ASEFGMVPGV RKDQGPALRA 250 260 270 280 290 300 AVSALRRQGG GVLNIPRGIY HFYPEGALNM SFHISNHDQP LIHPVCVPLA DLRNVRVEGN 310 320 330 340 350 360 GSLFLFHGKV VPLLVMDSEN VSINRLSVDY ERSWCTEARV VKTDDRFTEV EIDKKAYPYE 370 380 390 400 410 420 IRNNRFVFQG KGWEEGMGSC MAFEKGTGHI IANTSDIGWN GHVEPLGGSR LRLSWNLRQK 430 440 450 460 470 480 GIKPGDTLVL RNYNRPHPGC VVYRARKTSL NDVSLHQSSG MALLVQRSED FHMKGGGVMV 490 500 510 520 530 540 RKGTGRVHTA GADATHFSNT RGGIVVEKAL FEGMMDDAIN VHSTCLGVME VVDSHTLKCK 550 560 570 580 590 600 YMHRQAVGFE VFLPGEKIRF INGPTLEPGG TATVKTAVKK NSAEMVITVE EPLPSSVRAG 610 620 630 640 650 660 DAVENADFYP SVVFRNNIVR NNRARGSLFT TPERVLVEGN LFDHSSGSAI LLAGDAQGWY 670 680 690 700 710 720 ESGACHEVVI RKNTFINNLT SRYQFTNAII SIYPEVKQLD RQRDYYHRNV LIENNVFKTF 730 740 750 760 770 780 DVPLLFAIST DNLKFINNKV IYNDEFKGWG QKPFQFRRCA NILIKDNKVL PPRTWTLEDC 790 KLENTPSDQV RFGG

References

[1]

"Complete sequence of Akkermansia muciniphila ATCC BAA-835." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N. , Smidt H., Derrien M., Plugge C.M., Zoetendal E.G., de Vos W.M., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-835.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001071 Genomic DNA. Translation: ACD04318.1.
RefSeq	YP_001877099.1. NC_010655.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2UNU8.
Protein family/group databases
CAZy	CBM51. Carbohydrate-Binding Module Family 51. GH110. Glycoside Hydrolase Family 110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6275422.
GenomeReviews	Gene locus Amuc_0480 in contig CP001071_GR.
KEGG	amu:Amuc_0480.
PATRIC	20833580. VBIAkkMuc16855_0546.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LTWTPEV.
ProtClustDB	CLSK823424.
Family and domain databases
InterPro	IPR013222. Glyco_hyd_98_carb-bd. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 3 hits.
Pfam	PF08305. NPCBM. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 4 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLAB_AKKM8

Accession

Primary (citable) accession number: B2UNU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program