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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Akkermansia muciniphila (strain ATCC BAA-835 / Muc)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi493 – 4931MagnesiumUniRule annotation
Metal bindingi499 – 4991MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciAMUC349741:GHZ7-402-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Amuc_0382
OrganismiAkkermansia muciniphila (strain ATCC BAA-835 / Muc)
Taxonomic identifieri349741 [NCBI]
Taxonomic lineageiBacteriaVerrucomicrobiaVerrucomicrobiaeVerrucomicrobialesVerrucomicrobiaceaeAkkermansia
ProteomesiUP000001031 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 714714Polyribonucleotide nucleotidyltransferasePRO_1000147878Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi349741.Amuc_0382.

Structurei

3D structure databases

ProteinModelPortaliB2UNA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini559 – 61860KHUniRule annotationAdd
BLAST
Domaini628 – 69669S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2UNA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIHSVECNV GTNPITIETG KMARLADGAV VVRSGDTVVL VTVVSATKVK
60 70 80 90 100
EGQTFFPLSV EYKEKAAAAG MFPGGYFKRE GRPTEKEILT CRMTDRPLRP
110 120 130 140 150
MFPKGYFYDT QVITLLLSAD GENEPDILSI NGASAACVVS DLPFAEPVGA
160 170 180 190 200
VRVGRIDGQF VINPTNSQRE HSQLDLVFAG TKDQVIMIEG SANELPEEDF
210 220 230 240 250
IAALRVAQEN VKVICEKQEE LRAVCGKEKR AYELCLAKPE LLEIGYEIAG
260 270 280 290 300
DRIEEAIYAP SKVERQKKVG ALRDEVEAAI KERHPEATDF DVEQVFEYIQ
310 320 330 340 350
KKAFRISIME KDKRADGRAL KQLRPLTAEV NVLPPVVHGS AMFARGETMS
360 370 380 390 400
LCLATLAPME ERQYMDNYTG SVNEKRFILH YNFPPFSVGD TGRFGGQNRR
410 420 430 440 450
EIGHGALAER SIAPVVPGEQ EFPYAIRVSS EIMESNGSTS MASVCAGTMS
460 470 480 490 500
LLAAGVPLKR PVAGISVGLV TEQNDQHEIT SYKTLLDIIG SEDFYGDMDF
510 520 530 540 550
KLCGTSEGVT GYQLDLKLPG IPLSILEEAI HVAKAGRTDV LKVMNEAIAA
560 570 580 590 600
PAQMSPNAPR IETTKIPADR IGELIGPGGK NIKAIQAESG ADINIEEDGT
610 620 630 640 650
VHIYAAKQEG LDRALELVTR MFKTIEIGEL YTGKIVSTTT FGAFMEVLPG
660 670 680 690 700
KDGLIHISEL AEGRTAKTED VVSVGDVVTA KCIGIDDKGR VKMSIRAALR
710
DAKAAEAEAA GITE
Length:714
Mass (Da):77,480
Last modified:July 1, 2008 - v1
Checksum:i15FFBA34982322E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001071 Genomic DNA. Translation: ACD04221.1.
RefSeqiYP_001877002.1. NC_010655.1.

Genome annotation databases

EnsemblBacteriaiACD04221; ACD04221; Amuc_0382.
KEGGiamu:Amuc_0382.
PATRICi20833360. VBIAkkMuc16855_0436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001071 Genomic DNA. Translation: ACD04221.1.
RefSeqiYP_001877002.1. NC_010655.1.

3D structure databases

ProteinModelPortaliB2UNA9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349741.Amuc_0382.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD04221; ACD04221; Amuc_0382.
KEGGiamu:Amuc_0382.
PATRICi20833360. VBIAkkMuc16855_0436.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciAMUC349741:GHZ7-402-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Akkermansia muciniphila, a dedicated intestinal mucin degrader, and its use in exploring intestinal metagenomes."
    van Passel M.W., Kant R., Zoetendal E.G., Plugge C.M., Derrien M., Malfatti S.A., Chain P.S., Woyke T., Palva A., de Vos W.M., Smidt H.
    PLoS ONE 6:E16876-E16876(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-835 / Muc.

Entry informationi

Entry nameiPNP_AKKM8
AccessioniPrimary (citable) accession number: B2UNA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: April 29, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.