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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Akkermansia muciniphila (strain ATCC BAA-835 / Muc)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei214 – 2141ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAMUC349741:GHZ7-1943-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Amuc_1888
OrganismiAkkermansia muciniphila (strain ATCC BAA-835 / Muc)
Taxonomic identifieri349741 [NCBI]
Taxonomic lineageiBacteriaVerrucomicrobiaVerrucomicrobiaeVerrucomicrobialesAkkermansiaceaeAkkermansia
ProteomesiUP000001031 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 433433Glutamate--tRNA ligasePRO_0000367604Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi349741.Amuc_1888.

Structurei

3D structure databases

ProteinModelPortaliB2UN91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 2011"HIGH" regionAdd
BLAST
Motifi211 – 2155"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiCRERTEP.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 2 hits.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2UN91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLVRTRFAP SPTGYLHIGG ARTALFNWLF ARKMGGTFIL RIEDTDNARN
60 70 80 90 100
TEEATRAIFT GMEWLGLDWD EGPMKGGDCG PYFQSQRNDI YDAYFKKLQD
110 120 130 140 150
AGRVYEDEGA WRFRFDRSKP VTFHDLICGN ITIDYRDASN TPDMAIRRAD
160 170 180 190 200
GSYIFHFVNV VDDIEMKMTH VIRGEDHIMN TPKHIQLFEA FGVTPPVFAH
210 220 230 240 250
MPLILNQDGS KMSKRDVGAA LGTYPEEGFL PEGVMNFLAL LGWSPKDDTE
260 270 280 290 300
IFSPQELIGR FSLEAVNHSA AKFDITKCRW VNQQHIIALP AEEFALRARP
310 320 330 340 350
FCLKAGLPDS PILDAAIATV QTKVQTLAEV PDKIRFFFDL GMDPEAVSKV
360 370 380 390 400
QPEALELLFK LADRLEQEPE WDGHELIGVL KAFAKENGVK MGAVMFPARV
410 420 430
ALTGLSGGPD LSSVFSLLGR EETVRRIRSF SLD
Length:433
Mass (Da):48,607
Last modified:July 1, 2008 - v1
Checksum:i1C151550DCEB705F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001071 Genomic DNA. Translation: ACD05702.1.
RefSeqiWP_012420916.1. NC_010655.1.
YP_001878483.1. NC_010655.1.

Genome annotation databases

EnsemblBacteriaiACD05702; ACD05702; Amuc_1888.
KEGGiamu:Amuc_1888.
PATRICi20836854. VBIAkkMuc16855_2150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001071 Genomic DNA. Translation: ACD05702.1.
RefSeqiWP_012420916.1. NC_010655.1.
YP_001878483.1. NC_010655.1.

3D structure databases

ProteinModelPortaliB2UN91.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349741.Amuc_1888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD05702; ACD05702; Amuc_1888.
KEGGiamu:Amuc_1888.
PATRICi20836854. VBIAkkMuc16855_2150.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiCRERTEP.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciAMUC349741:GHZ7-1943-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 2 hits.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Akkermansia muciniphila, a dedicated intestinal mucin degrader, and its use in exploring intestinal metagenomes."
    van Passel M.W., Kant R., Zoetendal E.G., Plugge C.M., Derrien M., Malfatti S.A., Chain P.S., Woyke T., Palva A., de Vos W.M., Smidt H.
    PLoS ONE 6:E16876-E16876(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-835 / Muc.

Entry informationi

Entry nameiSYE_AKKM8
AccessioniPrimary (citable) accession number: B2UN91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: June 24, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.