B2UL12 (GLAA_AKKM8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-galactosidase A
EC=3.2.1.n1

Alternative name(s):
Exo-alpha-galactosidase A
EC=3.2.1.22

Gene names

Name:	glaA
Ordered Locus Names:	Amuc_1463

Organism

Akkermansia muciniphila (strain ATCC BAA-835) [Complete proteome] [HAMAP]

Taxonomic identifier

349741 [NCBI]

Taxonomic lineage

Bacteria › Verrucomicrobia › Verrucomicrobiae › Verrucomicrobiales › Verrucomicrobiaceae › Akkermansia

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alpha-galactosidase that specifically removes branched alpha-1,3-linked galactose residues present in blood group B antigens. Has no activity toward linear alpha-1,3-linked galactose residues By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. A subfamily. Contains 4 PbH1 repeats.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 596	575	Alpha-1,3-galactosidase A		PRO_5000370228
Regions
	Repeat	351 – 373	23	PbH1 1
	Repeat	482 – 504	23	PbH1 2
	Repeat	515 – 537	23	PbH1 3
	Repeat	547 – 569	23	PbH1 4

Sequences

	Sequence		Length	Mass (Da)
	B2UL12 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: FF5E844064C2FADD	FASTA	596	65,952
	10 20 30 40 50 60 MQNPVASLLF ILAMLTGPCP AADYPERTER TQSAGNHVWH IDPDKGNDGN PGTAPSTAWK 70 80 90 100 110 120 SMAPANRLIM ARGDTLVIHP GEHAVSLALM GEGSKQAPVT IRFMPGRHIF KHGALMTGKP 130 140 150 160 170 180 QISNTNDAPN EPKAMAIRLM EAKNIRLEGK PGATDILLEG KAIFVCMEHA ENVSLNGLGF 190 200 210 220 230 240 DYLHPTMGEF LVTEVEGDTM KATIPDGTLY TVKDGNLTWH GPGWEFRMGG YSKVFDSASG 250 260 270 280 290 300 TFQGRFDPGK TVIRELSPGK ISITFKEGSP TMKPGQSYQN RNTRRDCCGF FQYRSKNILW 310 320 330 340 350 360 NNCHIYYMHG MGVVSQFCEN IMFSHLKIAP RPRSLRTNSS WADNLHFSGC RGKIIVKDCV 370 380 390 400 410 420 LGASHDDAVN VHGTHLRIID RPAPNKITVR FMHPQTFGFD AFAAGDRIDY VSCNTLVPYA 430 440 450 460 470 480 SNTVSGVKQL NEKEIELTLQ HPNPGNIQPD DVVENVTWTP SVHISNTVCR HIPTRGFLLT 490 500 510 520 530 540 TRKPVLVERC RFEKTGMPAI LVEDDASGWY ESGVVRNMTI SRNTFIQCGE AVIQIVPHAP 550 560 570 580 590 RPEGDVHRNI TITGNTFDLK NGTAIRIRHT GDVKAEKNTF TKDGKKIPEE KAVDIR

References

[1]

"Complete sequence of Akkermansia muciniphila ATCC BAA-835." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N. , Smidt H., Derrien M., Plugge C.M., Zoetendal E.G., de Vos W.M., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-835.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001071 Genomic DNA. Translation: ACD05285.1.
RefSeq	YP_001878066.1. NC_010655.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2UL12.
Protein family/group databases
CAZy	GH110. Glycoside Hydrolase Family 110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6275708.
GenomeReviews	Gene locus Amuc_1463 in contig CP001071_GR.
KEGG	amu:Amuc_1463.
PATRIC	20835858. VBIAkkMuc16855_1661.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LKLRFMH.
ProtClustDB	CLSK823424.
Family and domain databases
InterPro	IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 4 hits.
SMART	SM00710. PbH1. 4 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLAA_AKKM8

Accession

Primary (citable) accession number: B2UL12

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program