B2UKT4 (SYA_AKKM8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Amuc_1383

Organism

Akkermansia muciniphila (strain ATCC BAA-835) [Complete proteome] [HAMAP]

Taxonomic identifier

349741 [NCBI]

Taxonomic lineage

Bacteria › Verrucomicrobia › Verrucomicrobiae › Verrucomicrobiales › Verrucomicrobiaceae › Akkermansia

Protein attributes

Sequence length	942 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 942	942	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347482
Sites
	Metal binding	586	1	Zinc Potential
	Metal binding	590	1	Zinc Potential
	Metal binding	695	1	Zinc Potential
	Metal binding	699	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	B2UKT4 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: ED53F46EB999D0D9	FASTA	942	103,645
	10 20 30 40 50 60 MMTATEIRQS FLDFFREKQH TVVPSASLMP QSPGLLFTNA GMNQFVPYFL GVWTPPWTPA 70 80 90 100 110 120 RATDTQKCIR AGGKHNDLED VGYDSYHHTF FEMLGNWSFG DYFKREAIRW AWELVVERWG 130 140 150 160 170 180 FPAERLYATV YAPDKSKGDP GEFDREAWDF WAELFRSRGL DPDVHIVHGN VKDNFWMMGE 190 200 210 220 230 240 TGPCGPCSEL HVDLTPEGNT KGSLVNKDSD QCIEIWNLVF IQYNAESDGS MRNLPACHVD 250 260 270 280 290 300 TGMGFERACS IMQCTNGFKD FSRKPSNYAT DVFRPLFDRL EVLSGRKYAD VYPAPGSKRV 310 320 330 340 350 360 DAEDGTLQEA IAFRVIADHL RTLSFSIADG ILPGNNGRNY VLRRILRRAV RYGRRLGFTQ 370 380 390 400 410 420 PFLAELVDTL VESFGQVFPE LAARATTVKE VLNREEASFN ETLDRGLELF DAETASAGKV 430 440 450 460 470 480 SGEFAFKLYD TYGFPIDLTA LLAEERGLDI DMERFNRLME EQRERARAAR KSEVVRALDL 490 500 510 520 530 540 KTDAVTEFTG YDVDECAATV LEVSRQGDSL FIITDKTPFY AEMGGQVSDA GLIEIGGESY 550 560 570 580 590 600 HVMAVQQIGN ARAHVVEARP GLEVKPGDRV HLSIDAERRR RIEAHHTATH LLHCALHQVV 610 620 630 640 650 660 SPDAAQQGSF VSEDRLRFDF NSSAVSPDQL RLIEEKVNGW IEESLPVHCT ERAYADVKGN 670 680 690 700 710 720 AAIAQFFGDK YGDVVRVVQV GGCRDGLDGV SMEFCGGTHI ANTKNIGLFK IKSEGAIASG 730 740 750 760 770 780 VRRIEAMTGD AALEMIRQHV VAKSLEIAKA VEKIKEVNYE LADMGLEQVP VPTIEGKPGL 790 800 810 820 830 840 TALGASDIRT VNDSLARFDA SVEHFKQTAL DAEKKLKKAR AGQSAAKADA LLNEWLSDAP 850 860 870 880 890 900 SSLIQVAEGA GELLQELLNG LKKRQYAGAA FLLCVDSSSL LLGAYCGKDA IADGLSAGDM 910 920 930 940 IREVAALAGG KGGGRADQAR GSAPQDADPQ ALAAAARNII NG

References

[1]

"Complete sequence of Akkermansia muciniphila ATCC BAA-835." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N. , Smidt H., Derrien M., Plugge C.M., Zoetendal E.G., de Vos W.M., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-835.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001071 Genomic DNA. Translation: ACD05207.1.
RefSeq	YP_001877988.1. NC_010655.1.
3D structure databases
ProteinModelPortal	B2UKT4.
ModBase	Search...
Protein-protein interaction databases
STRING	B2UKT4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6274625.
GenomeReviews	Gene locus Amuc_1383 in contig CP001071_GR.
KEGG	amu:Amuc_1383.
PATRIC	20835640. VBIAkkMuc16855_1557.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG354397.
OMA	VILEMES.
ProtClustDB	PRK00252.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_AKKM8

Accession

Primary (citable) accession number: B2UKT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program