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Protein

DNA polymerase IV

Gene

dinB

Organism
Akkermansia muciniphila (strain ATCC BAA-835 / Muc)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101MagnesiumUniRule annotation
Sitei15 – 151Substrate discriminationUniRule annotation
Metal bindingi104 – 1041MagnesiumUniRule annotation
Active sitei105 – 1051UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciAMUC349741:GHZ7-1371-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:Amuc_1330
OrganismiAkkermansia muciniphila (strain ATCC BAA-835 / Muc)
Taxonomic identifieri349741 [NCBI]
Taxonomic lineageiBacteriaVerrucomicrobiaVerrucomicrobiaeVerrucomicrobialesAkkermansiaceaeAkkermansia
Proteomesi
  • UP000001031 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359DNA polymerase IVPRO_1000163996Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi349741.Amuc_1330.

Structurei

3D structure databases

ProteinModelPortaliB2UKN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 186181UmuCUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation
Contains 1 umuC domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQ3. Bacteria.
COG0389. LUCA.
HOGENOMiHOG000082707.
KOiK02346.
OMAiMQIREIF.
OrthoDBiEOG6FZ4D8.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2UKN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQRKIIHVD MDAFYASIEQ RDHPEYRGKP IAVGRPEMRG VVAAASYEAR
60 70 80 90 100
RFGVRSAMPS MKALKLCPHL IFTRNRMDVY KAVSAQIHAI FHRYTDLVEP
110 120 130 140 150
LSLDEAFLDV TENKPGIPLA VDIARRIKKE IRRELHLTAS AGVSYNKFLA
160 170 180 190 200
KIASDYRKPD GLFTIHPSRA EKFIAALPIE AFWGVGHATA ERMRALSITN
210 220 230 240 250
GAQLRARDKD FLVRHFGKTG AIFYNFARGV DDRPVEPSRM RKSVGCEETY
260 270 280 290 300
RENVTRAEAL EQRLPLLAEE LAGRLARSGF RGNTLTLKVK FPDFVQKTRC
310 320 330 340 350
ATVPEILTEK EGILPLARTL MEELDSGDRT FRLLGLSVSH PQEEQRQGIW

EQLWLELEY
Length:359
Mass (Da):40,891
Last modified:July 1, 2008 - v1
Checksum:i0547AE2C1D4410E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001071 Genomic DNA. Translation: ACD05154.1.
RefSeqiWP_012420369.1. NC_010655.1.

Genome annotation databases

EnsemblBacteriaiACD05154; ACD05154; Amuc_1330.
KEGGiamu:Amuc_1330.
PATRICi20835538. VBIAkkMuc16855_1507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001071 Genomic DNA. Translation: ACD05154.1.
RefSeqiWP_012420369.1. NC_010655.1.

3D structure databases

ProteinModelPortaliB2UKN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349741.Amuc_1330.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD05154; ACD05154; Amuc_1330.
KEGGiamu:Amuc_1330.
PATRICi20835538. VBIAkkMuc16855_1507.

Phylogenomic databases

eggNOGiENOG4105CQ3. Bacteria.
COG0389. LUCA.
HOGENOMiHOG000082707.
KOiK02346.
OMAiMQIREIF.
OrthoDBiEOG6FZ4D8.

Enzyme and pathway databases

BioCyciAMUC349741:GHZ7-1371-MONOMER.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Akkermansia muciniphila, a dedicated intestinal mucin degrader, and its use in exploring intestinal metagenomes."
    van Passel M.W., Kant R., Zoetendal E.G., Plugge C.M., Derrien M., Malfatti S.A., Chain P.S., Woyke T., Palva A., de Vos W.M., Smidt H.
    PLoS ONE 6:E16876-E16876(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-835 / Muc.

Entry informationi

Entry nameiDPO4_AKKM8
AccessioniPrimary (citable) accession number: B2UKN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: December 9, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.