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Protein

Homogentisate 1,2-dioxygenase

Gene

hmgA

Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the catabolism of homogentisate (2,5-dihydroxyphenylacetate or 2,5-OH-PhAc), a central intermediate in the degradation of phenylalanine and tyrosine. Catalyzes the oxidative ring cleavage of the aromatic ring of homogentisate to yield maleylacetoacetate.UniRule annotation

Catalytic activityi

Homogentisate + O2 = 4-maleylacetoacetate.UniRule annotation

Cofactori

Fe cationUniRule annotation

Pathwayi: L-phenylalanine degradation

This protein is involved in step 4 of the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Homogentisate 1,2-dioxygenase (hmgA)
  5. no protein annotated in this organism
  6. no protein annotated in this organism
This subpathway is part of the pathway L-phenylalanine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine, the pathway L-phenylalanine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei302Proton acceptorUniRule annotation1
Metal bindingi345IronUniRule annotation1
Metal bindingi351IronUniRule annotation1
Binding sitei360homogentisateUniRule annotation1
Metal bindingi381IronUniRule annotation1
Binding sitei381homogentisateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processPhenylalanine catabolism, Tyrosine catabolism
LigandIron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00139; UER00339

Names & Taxonomyi

Protein namesi
Recommended name:
Homogentisate 1,2-dioxygenaseUniRule annotation (EC:1.13.11.5UniRule annotation)
Short name:
HGDOUniRule annotation
Alternative name(s):
Homogentisate oxygenaseUniRule annotation
Homogentisic acid oxidaseUniRule annotation
HomogentisicaseUniRule annotation
Gene namesi
Name:hmgAUniRule annotation
Ordered Locus Names:Rpic_4534
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000002566 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001198471 – 448Homogentisate 1,2-dioxygenaseAdd BLAST448

Proteomic databases

PRIDEiB2UJ73

Interactioni

Subunit structurei

Hexamer; dimer of trimers.UniRule annotation

Protein-protein interaction databases

STRINGi402626.Rpic_4534

Structurei

3D structure databases

SMRiB2UJ73
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the homogentisate dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F1J Bacteria
COG3508 LUCA
HOGENOMiHOG000139824
KOiK00451
OMAiHGNYTPY

Family and domain databases

Gene3Di2.60.120.10, 1 hit
HAMAPiMF_00334 Homogentis_dioxygen, 1 hit
InterProiView protein in InterPro
IPR005708 Homogentis_dOase
IPR022950 Homogentis_dOase_bac
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR11056 PTHR11056, 1 hit
PfamiView protein in Pfam
PF04209 HgmA, 1 hit
SUPFAMiSSF51182 SSF51182, 1 hit
TIGRFAMsiTIGR01015 hmgA, 1 hit

Sequencei

Sequence statusi: Complete.

B2UJ73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMLAPTAKN AFTPASLDRP AYQSGFGNEF STEALPGALP HGQNSPQKAP
60 70 80 90 100
YGLYAEQISG TAFTAPRAHN RRSWLYRIRP GAVHLPFEAM AQGRFHSHFN
110 120 130 140 150
EVPPSPNQLR WDPLPAPAAG TDFIDGIVTF AGNGGPDAQT GCGIHLYAAN
160 170 180 190 200
ADMTDRFFYN ADGELLIVPQ QGRLRLLTEM GVVDVEPLEI AVIPRGVRFR
210 220 230 240 250
VELPDGDARG YICENFGALF RLPDLGVIGS NGLANPRDFL TPHAWYEDRE
260 270 280 290 300
GAFELVAKFQ GSLWTAKIGH SPLDVVAWHG NLAPYKYDLR LFNTIGSISY
310 320 330 340 350
DHPDPSIFLV LQSPSATPGV DTIDFVIFPP RWLAAENTFR PPWFHRNVAS
360 370 380 390 400
EFMGLIQGVY DAKAEGFVPG GASLHNCMSG HGPDADTFEK ASNSDTTKPH
410 420 430 440
KVDATMAFMF ETPAVIRPTR FAAESAQLQA KYFECWQGLK KHFDPSKR
Length:448
Mass (Da):49,276
Last modified:July 1, 2008 - v1
Checksum:i4087D2B86E626C5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001069 Genomic DNA Translation: ACD29624.1
RefSeqiWP_012430499.1, NC_010678.1

Genome annotation databases

EnsemblBacteriaiACD29624; ACD29624; Rpic_4534
GeneIDi6285987
KEGGirpi:Rpic_4534

Similar proteinsi

Entry informationi

Entry nameiHGD_RALPJ
AccessioniPrimary (citable) accession number: B2UJ73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: May 23, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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