B2UGQ9 (B2UGQ9_RALPJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase HAMAP-Rule MF_01374
EC=3.1.2.6 HAMAP-Rule MF_01374

Alternative name(s):
Glyoxalase II HAMAP-Rule MF_01374

Gene names

Name:	gloB HAMAP-Rule MF_01374
Ordered Locus Names:	Rpic_2039 EMBL ACD27173.1

Organism

Ralstonia pickettii (strain 12J) [Complete proteome] [HAMAP] EMBL ACD27173.1

Taxonomic identifier

402626 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia › Burkholderia pickettii

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. SAAS SAAS017782 HAMAP-Rule MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. SAAS SAAS017782 HAMAP-Rule MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. SAAS SAAS017782 HAMAP-Rule MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP-Rule MF_01374
Subunit structure	Monomer By similarity. HAMAP-Rule MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. HAMAP-Rule MF_01374

Ontologies

Keywords
Ligand	Metal-binding SAAS SAAS017782 HAMAP-Rule MF_01374 Zinc SAAS SAAS017782 HAMAP-Rule MF_01374
Molecular function	Hydrolase SAAS SAAS017782 HAMAP-Rule MF_01374 EMBL ACD27173.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutathione biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	63	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	65	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	67	1	Zinc 2 By similarity HAMAP-Rule MF_01374
	Metal binding	68	1	Zinc 2 By similarity HAMAP-Rule MF_01374
	Metal binding	128	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	152	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	152	1	Zinc 2 By similarity HAMAP-Rule MF_01374
	Metal binding	190	1	Zinc 2 By similarity HAMAP-Rule MF_01374

Sequences

	Sequence		Length	Mass (Da)
	B2UGQ9 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 739D8683B8C0E0A0	FASTA	279	29,602
	10 20 30 40 50 60 MVQGGVPRVV ALEVEAIAAF TDNYIWALHD GRVAAVVDPG DAQPVLRFLK ERGLALGAIV 70 80 90 100 110 120 ITHHHGDHVG GVRELLAAYP ENPEGAPLQV IGPADESIPC RTQAVREGDV VTLTQPAATF 130 140 150 160 170 180 RVIDVPGHTS GHVAYVGRLQ GNAASASLFC GDTLFATGCG RLFEGTPAQM RASLAKLAAL 190 200 210 220 230 240 APDTRVYCAH EYTASNVRFA RAVEPGNADL AAWEDEVAAL RAEGRSTVPT TVGHERATNP 250 260 270 FMRSDDANVT QAVARHAGID ADDPVAVFAA LRQWKNDFR

References

[1]

"Complete sequence of chromosome 1 of Ralstonia pickettii 12J." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marsh T., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12J.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001068 Genomic DNA. Translation: ACD27173.1.
RefSeq	YP_001899605.1. NC_010682.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	402626.Rpic_2039.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD27173; ACD27173; Rpic_2039.
GeneID	6288456.
KEGG	rpi:Rpic_2039.
PATRIC	20254892. VBIRalPic63053_3190.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HOG000058041.
KO	K01069.
OMA	HILTTHH.
ProtClustDB	CLSK896990.
Enzyme and pathway databases
BioCyc	RPIC402626:GH94-2157-MONOMER.
UniPathway	UPA00619; UER00676.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2.
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
PIRSF	PIRSF005457. Glx. 1 hit.
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B2UGQ9_RALPJ

Accession

Primary (citable) accession number: B2UGQ9

Entry history

Integrated into UniProtKB/TrEMBL:	July 1, 2008
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)