B2UG80 (GCST_RALPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	Rpic_3487

Organism

Ralstonia pickettii (strain 12J) [Complete proteome] [HAMAP]

Taxonomic identifier

402626 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia › Burkholderia pickettii

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP-Rule MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP-Rule MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Aminomethyltransferase HAMAP-Rule MF_00259		PRO_1000114108

Sequences

	Sequence		Length	Mass (Da)
	B2UG80 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 6E8CAE7C9F89D7CA	FASTA	375	40,148
	10 20 30 40 50 60 MTLQHTPLNA IHRSLGARMV DFGGWDMPVN YGSQIEEHHA VRQDAGVFDV SHMCVVDLTG 70 80 90 100 110 120 ARVRDFLRGL LANNVDKLQT PGKALYSCML NPKGGVIDDL IVYFFREDWF RLVVNAGTAP 130 140 150 160 170 180 TDLEWIVAQN AAAGTDVTIT PRRSDNNAGA EPLGILAVQG PNARAKTYAA LPGSQAVGDA 190 200 210 220 230 240 LKPFNAGFVT VENVGEIMVA RTGYTGEDGF ELVVPAAQIA GVWERLLQAG VRPAGLGARD 250 260 270 280 290 300 TLRLEAGMNL YGQDMDEHVS PLDAGLAWTV DLQSERDFTG KAALQAGGQR EQFVGLILRD 310 320 330 340 350 360 KGGVLRAHQK VITAAGDGEI TSGTFSPSLS LSIALARLPK EVPVGTDVQV EIRDRKLTAT 370 VVKLPFVRNG KALVS

References

[1]

"Complete sequence of chromosome 1 of Ralstonia pickettii 12J." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marsh T., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12J.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001068 Genomic DNA. Translation: ACD28607.1.
RefSeq	YP_001901039.1. NC_010682.1.
3D structure databases
ProteinModelPortal	B2UG80.
SMR	B2UG80. Positions 5-373.
ModBase	Search...
Protein-protein interaction databases
STRING	402626.Rpic_3487.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD28607; ACD28607; Rpic_3487.
GeneID	6286303.
KEGG	rpi:Rpic_3487.
PATRIC	20257813. VBIRalPic63053_4621.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0404.
HOGENOM	HOG000239381.
KO	K00605.
OMA	WSIAWEP.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	RPIC402626:GH94-3634-MONOMER.
Family and domain databases
Gene3D	3.30.1360.120. 2 hits.
HAMAP	MF_00259. GcvT.
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. IPR027266. TrmE/GcvT_dom1. [Graphical view]
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_RALPJ

Accession

Primary (citable) accession number: B2UG80

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program