B2UFT7 (B2UFT7_RALPJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108 RuleBase RU003490
EC=2.7.7.60 HAMAP MF_00108 RuleBase RU003490

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	ispD HAMAP MF_00108
Ordered Locus Names:	Rpic_1903

Organism

Ralstonia pickettii (strain 12J) [Complete proteome] [HAMAP]

Taxonomic identifier

402626 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004138
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108 RuleBase RU004393

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393
Molecular function	Nucleotidyltransferase HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393 EMBL ACD27038.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	22	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	29	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	170	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	226	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	B2UFT7 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 437C19372F27B267	FASTA	252	26,459
	10 20 30 40 50 60 MSISHPGRRR FALIPSAGTG SRAGGDLPKQ YQVIAGRPML WHTAQAFLAS PEIDTVLVVT 70 80 90 100 110 120 TPGAQPLVQR FPEGGFNAPK LVEVDCGGDS RHASVTHGLA ALRGLGAHDD DWVLVHDAAR 130 140 150 160 170 180 PGLTPALVAR LVAAVEAGGT QAAGGILALP VADTLKRADA AQHINATVPR DGLWQAQTPQ 190 200 210 220 230 240 MFPLGLLERA LREALLQQRI VTDEASAIEA AGHPPLLVPG ALRNFKVTYS DDFALAEAIL 250 AQRAQPNEAS QA

References

[1]

"Complete sequence of chromosome 1 of Ralstonia pickettii 12J." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marsh T., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001068 Genomic DNA. Translation: ACD27038.1.
RefSeq	YP_001899470.1. NC_010682.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2UFT7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6287755.
GenomeReviews	Gene locus Rpic_1903 in contig CP001068_GR.
KEGG	rpi:Rpic_1903.
PATRIC	20254610. VBIRalPic63053_3055.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	ALSIVHT.
ProtClustDB	PRK00155.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B2UFT7_RALPJ

Accession

Primary (citable) accession number: B2UFT7

Entry history

Integrated into UniProtKB/TrEMBL:	July 1, 2008
Last sequence update:	July 1, 2008
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)