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B2UF62 (B2UF62_RALPJ) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase HAMAP-Rule MF_00419
Short name=FGAM synthase HAMAP-Rule MF_00419
Short name=FGAMS HAMAP-Rule MF_00419
EC=6.3.5.3 HAMAP-Rule MF_00419
Alternative name(s):
Formylglycinamide ribotide amidotransferase HAMAP-Rule MF_00419
Formylglycinamide ribotide synthetase HAMAP-Rule MF_00419
Gene names
Name:purL HAMAP-Rule MF_00419
Ordered Locus Names:Rpic_1858 EMBL ACD26994.1
OrganismRalstonia pickettii (strain 12J) [Complete proteome] [HAMAP] EMBL ACD26994.1
Taxonomic identifier402626 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia ›

Protein attributes

Sequence length1368 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. SAAS SAAS010073 HAMAP-Rule MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. SAAS SAAS010073 HAMAP-Rule MF_00419

Subunit structure

Monomer By similarity. SAAS SAAS010073 HAMAP-Rule MF_00419

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00419.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain. SAAS SAAS010073 HAMAP-Rule MF_00419

In the N-terminal section; belongs to the FGAMS family. HAMAP-Rule MF_00419

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain1115 – 1368254Glutamine amidotransferase type-1 By similarity HAMAP-Rule MF_00419
Nucleotide binding329 – 34012ATP By similarity HAMAP-Rule MF_00419

Sites

Active site12081Nucleophile By similarity HAMAP-Rule MF_00419
Active site13291 By similarity HAMAP-Rule MF_00419
Active site13311 By similarity HAMAP-Rule MF_00419

Sequences

Sequence LengthMass (Da)Tools
B2UF62 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 23C402FEAF547E51

FASTA1,368147,453
        10         20         30         40         50         60 
MAHFSCFPGA LALSAFRQQR LLSTLKRIDP DIDGVSAQYL HFVAADTPLS ADESARVQAL 

        70         80         90        100        110        120 
LTYGSPAPAD VEGDRFVVIP RFGTISPWAS KATEIARHCA LPQIHRIERG VEFTVTCKKG 

       130        140        150        160        170        180 
LIRSLTGAAK HLDDATRAAV AEHLHDRMTE TVIDSREAGY GLFDVLPAKA LRFVDIAGGQ 

       190        200        210        220        230        240 
DAGRRALEAA NVEMGLALSD DEIDYLVDAY RKLERNPTDV ELMMFAQANS EHCRHKIFNA 

       250        260        270        280        290        300 
DWTIDGETQD KSLFAMIRNT HQLAPQGTIV AYSDNAAVME GGMAERWFAH AGTEGETGVP 

       310        320        330        340        350        360 
QYGRREALTH TLMKVETHNH PTAISPFPGA STGAGGEIRD EGATGRGAKP KAGLTGFTVS 

       370        380        390        400        410        420 
NLLLPEAVQS WENARDTAQP VAHRNPDEAA PGPVGKPDRI ASPLQIMIEG PIGGAAFNNE 

       430        440        450        460        470        480 
FGRPNLGGYF RVYEQNVGGT VRGYHKPIMI AGGIGNIDAG HTHKHGLPAG TLLIQLGGPG 

       490        500        510        520        530        540 
MRIGMGGGAA SSMATGTNTA DLDFDSVQRG NPEMQRRAQE VINACWALGE DNPILSIHDV 

       550        560        570        580        590        600 
GAGGISNAFP ELVDGAGKGA RFDLRQVHLE ESGLSPAEIW CNESQERYTL AIAPGDFPRF 

       610        620        630        640        650        660 
QAMCERERAP FSVVGFATDE QQLQVVDSDT AADVAEHFPV NMPMDVLLGK PPRMHRDVRR 

       670        680        690        700        710        720 
VAQELPEVDV TGLDLEVVAR DVLRHPTVAS KSFLITIGDR TVGGLNTRDQ MVGPWQVPVA 

       730        740        750        760        770        780 
DVAVTTLDYK GYAGEAMTMG ERTPLAVINA PASGRMAIGE SITNIAAAPI DSLTQLKLSA 

       790        800        810        820        830        840 
NWMAACGVNG EDARLYDTVK AVGMELCPAL GISIPVGKDS LSMRTKWEDE VAAKEVVAPV 

       850        860        870        880        890        900 
SLIVSAFAPV TDVRKTLTPQ LKQVESANAP ADSTLILIDL GRGKNRMGGS ILAQVTEQIG 

       910        920        930        940        950        960 
NSVPDVDDAE DLKRFFAAIQ QLNASGSLLA YHDRSDGGLW ATVCEMAFAG HCGVSINVDM 

       970        980        990       1000       1010       1020 
LTLDGAHESD YGDAKNWAQQ VSGRRADLTL RALFAEELGA VIQVPLAQRD AVFAVLREHG 

      1030       1040       1050       1060       1070       1080 
LAGCSHVIGA PNANDHIEVW RDAKKVFSAS RIELQRTWTD VSWRIASLRD NPECTQSEYD 

      1090       1100       1110       1120       1130       1140 
RILDAEDPGI SPKLTFDIAE DVAAPFIATG ARPRMAILRE QGVNSQIEMA YAMDKAGFDT 

      1150       1160       1170       1180       1190       1200 
YDVHMSDLLA GRTNLASFKG FVACGGFSYG DVLGAGEGWA KTILFNSMLA EQFAAFFNRT 

      1210       1220       1230       1240       1250       1260 
DSIALGVCNG CQMMANLAPI IPGAGAWPKF TRNQSEQYEG RLVTVQVEAS PAIFYAGMEG 

      1270       1280       1290       1300       1310       1320 
SRIPIVVAHG EGYADFSQQG DIDKVAVGLR YVDNRGEVTQ TYPLNPNGSP QGIASVTTHD 

      1330       1340       1350       1360 
GRFTALMPHP ERVFRAVQMS WHPKEWEAAG DGSSPWMRMF RNARKWMA

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References

[1]"Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 12J.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001068 Genomic DNA. Translation: ACD26994.1.
RefSeqYP_001899426.1. NC_010682.1.

3D structure databases

ProteinModelPortalB2UF62.
ModBaseSearch...

Protein-protein interaction databases

STRING402626.Rpic_1858.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD26994; ACD26994; Rpic_1858.
GeneID6289855.
KEGGrpi:Rpic_1858.
PATRIC20254514. VBIRalPic63053_3010.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0046.
HOGENOMHOG000261359.
KOK01952.
OMATVKAVGM.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycRPIC402626:GH94-1967-MONOMER.
UniPathwayUPA00074; UER00128.

Family and domain databases

HAMAPMF_00419. PurL_1.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB2UF62_RALPJ
AccessionPrimary (citable) accession number: B2UF62
Entry history
Integrated into UniProtKB/TrEMBL: July 1, 2008
Last sequence update: July 1, 2008
Last modified: May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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