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B2UE01

- LIPA_RALPJ

UniProt

B2UE01 - LIPA_RALPJ

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Protein
Lipoyl synthase
Gene
lipA, Rpic_0178
Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi80 – 801Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi91 – 911Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi106 – 1061Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi110 – 1101Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi113 – 1131Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRPIC402626:GH94-177-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Rpic_0178
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000002566: Chromosome 1

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Lipoyl synthaseUniRule annotation
PRO_1000099623Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi402626.Rpic_0178.

Structurei

3D structure databases

ProteinModelPortaliB2UE01.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiTIRAVRH.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B2UE01-1 [UniParc]FASTAAdd to Basket

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MTDSAAGATE VATPATPSNK PYDATAKQKS LDKTARIPIK IVPAEKLKKP    50
DWIRVRAATG NSRFYEIKDI LRANNLVTVC EEASCPNIGE CFGKGTATFM 100
IMGDKCTRRC PFCDVGHGRP DPLDVNEPEN LAKTIAELKL NYVVITSVDR 150
DDLRDGGAQH YVDCISRTRA LSPATRIEVL VPDFRGRLEK ALDILQECPP 200
DVMNHNLETV PRLYKQARPG ADYAHSLKLL KDFKARNPNV PTKSGLMVGL 250
GETDEEILEV MRDMREHDID MLTIGQYLAP SGHHLPVLRY VHPDTFKMFE 300
EKAYEMGFTH AAVGAMVRSS YHADQQAHEA GVV 333
Length:333
Mass (Da):36,988
Last modified:July 1, 2008 - v1
Checksum:i58FD7993B4BD392E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001068 Genomic DNA. Translation: ACD25341.1.
RefSeqiYP_001897773.1. NC_010682.1.

Genome annotation databases

EnsemblBacteriaiACD25341; ACD25341; Rpic_0178.
GeneIDi6289347.
KEGGirpi:Rpic_0178.
PATRICi20251224. VBIRalPic63053_1381.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001068 Genomic DNA. Translation: ACD25341.1 .
RefSeqi YP_001897773.1. NC_010682.1.

3D structure databases

ProteinModelPortali B2UE01.
ModBasei Search...

Protein-protein interaction databases

STRINGi 402626.Rpic_0178.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD25341 ; ACD25341 ; Rpic_0178 .
GeneIDi 6289347.
KEGGi rpi:Rpic_0178.
PATRICi 20251224. VBIRalPic63053_1381.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi TIRAVRH.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci RPIC402626:GH94-177-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 12J.

Entry informationi

Entry nameiLIPA_RALPJ
AccessioniPrimary (citable) accession number: B2UE01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3
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