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Protein

Lipoyl synthase

Gene

lipA

Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi80Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi85Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi91Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi106Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi110Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi113Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:Rpic_0178
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000002566 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000996231 – 333Lipoyl synthaseAdd BLAST333

Interactioni

Protein-protein interaction databases

STRINGi402626.Rpic_0178

Structurei

3D structure databases

ProteinModelPortaliB2UE01
SMRiB2UE01
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0G Bacteria
COG0320 LUCA
HOGENOMiHOG000235997
KOiK03644
OMAiPYCDIDF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

B2UE01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDSAAGATE VATPATPSNK PYDATAKQKS LDKTARIPIK IVPAEKLKKP
60 70 80 90 100
DWIRVRAATG NSRFYEIKDI LRANNLVTVC EEASCPNIGE CFGKGTATFM
110 120 130 140 150
IMGDKCTRRC PFCDVGHGRP DPLDVNEPEN LAKTIAELKL NYVVITSVDR
160 170 180 190 200
DDLRDGGAQH YVDCISRTRA LSPATRIEVL VPDFRGRLEK ALDILQECPP
210 220 230 240 250
DVMNHNLETV PRLYKQARPG ADYAHSLKLL KDFKARNPNV PTKSGLMVGL
260 270 280 290 300
GETDEEILEV MRDMREHDID MLTIGQYLAP SGHHLPVLRY VHPDTFKMFE
310 320 330
EKAYEMGFTH AAVGAMVRSS YHADQQAHEA GVV
Length:333
Mass (Da):36,988
Last modified:July 1, 2008 - v1
Checksum:i58FD7993B4BD392E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA Translation: ACD25341.1
RefSeqiWP_004633801.1, NC_010682.1

Genome annotation databases

EnsemblBacteriaiACD25341; ACD25341; Rpic_0178
GeneIDi6289347
KEGGirpi:Rpic_0178

Similar proteinsi

Entry informationi

Entry nameiLIPA_RALPJ
AccessioniPrimary (citable) accession number: B2UE01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: May 23, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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