B2UDV1 (ACEK_RALPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Rpic_0128

Organism

Ralstonia pickettii (strain 12J) [Complete proteome] [HAMAP]

Taxonomic identifier

402626 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 611	611	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_1000133275
Regions
	Nucleotide binding	328 – 334	7	ATP By similarity
Sites
	Active site	384	1	By similarity
	Binding site	349	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2UDV1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 8D5CA3021ABF5557	FASTA	611	71,186
	10 20 30 40 50 60 MSHFPKLLSS QIAFDVARTM LDGFDKHYRL FREVSVQAKT AFETGDWAGL QQLQRDRIAY 70 80 90 100 110 120 YDERVREASV ILEDEYDAEN IDDEIWRQIK LHFIGLLTNH HQPECAETFF NSVCTRILHR 130 140 150 160 170 180 SYFNNDFIFV RPAISTEYIE NEESPTKPTY RAYYPGTRDG LGACFERIVH NFQLNAPFED 190 200 210 220 230 240 LERDIGYVVR AVGEHFGDFR IAPNFQIHVL SSLFFRNKAA YIIGRVINGD KTFPLAVPIL 250 260 270 280 290 300 RNAAGQLSLD TVLLRQEQLL ILFSFTHSYF MVDMEIPSAY VTFLRDLMPR KPRAEIYTSL 310 320 330 340 350 360 GLQKQGKNLF YRDFLHHLQH SSDKFIIAPG IKGLVMLVFT LPSYPYVFKV IKDYFPPPKE 370 380 390 400 410 420 TTRELIKSKY QLVKRHDRVG RMADTLEYSD VAFPLSRFDD DLIKELEKHA PSMVEYQRSE 430 440 450 460 470 480 DGGEEIVIRH VYIERRMTPL NIWLHEGTDA QVEHGIIEYG NAIKELIAAN IFPGDMLYKN 490 500 510 520 530 540 FGVTRHGRVV FYDYDEIEYL TDCNIRTVPE PRTEEEEMSG EVWYNVGPHD IFPETYRTFL 550 560 570 580 590 600 LGDPRVREAF LKHHADFFEA SMWQGYKERL LSGQMHDFYA YDAAERFVNR YGASAPDNAA 610 QDNTTLQRAA A

References

[1]

"Complete sequence of chromosome 1 of Ralstonia pickettii 12J." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marsh T., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12J.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001068 Genomic DNA. Translation: ACD25291.1.
RefSeq	YP_001897723.1. NC_010682.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2UDV1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6289796.
GenomeReviews	Gene locus Rpic_0128 in contig CP001068_GR.
KEGG	rpi:Rpic_0128.
PATRIC	20251120. VBIRalPic63053_1329.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298593.
OMA	RMTPLNI.
ProtClustDB	PRK02946.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_RALPJ

Accession

Primary (citable) accession number: B2UDV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program