SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B2UDA1

- BIOB_RALPJ

UniProt

B2UDA1 - BIOB_RALPJ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Biotin synthase

Gene
bioB, Rpic_0117
Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi80 – 801Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi117 – 1171Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi148 – 1481Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi208 – 2081Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi280 – 2801Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRPIC402626:GH94-116-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Rpic_0117
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000002566: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Biotin synthaseUniRule annotationPRO_0000381575Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi402626.Rpic_0117.

Structurei

3D structure databases

ProteinModelPortaliB2UDA1.
SMRiB2UDA1. Positions 26-330.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B2UDA1-1 [UniParc]FASTAAdd to Basket

« Hide

MQSTPLNFVP NAAKAPVTPG QAPDARWSRE AIEALFALPF NDLLFQAQQV    50
HRANFDANAV QLSTLLSIKT GGCPEDCSYC PQSARYDTGV EAEKLMPIDA 100
VLEAASRAKQ NGASRFCMGA AWRNPKPHQL DAVADMVRGV KAMGLETCVT 150
LGMLKQEQAA QLKEAGLDYY NHNLDTAPEF YGEIITTRTY QDRLDTLEHV 200
RDAGINVCCG GIVGLGESVH ERAGLIAELA NMEPYPDSVP INNLVKVEGT 250
PLAGNEELDP FDFVRTIAVA RITMPKAMVR LSAGREAMDD ALQALCFMAG 300
ANSIFYGEKL LTTDNPEADA DRKLLARLGM RVEVQDHMHH ESHVGAQSSH 350
CHIDITPAD 359
Length:359
Mass (Da):39,076
Last modified:July 1, 2008 - v1
Checksum:i8A21E191E3C7B129
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001068 Genomic DNA. Translation: ACD25280.1.
RefSeqiYP_001897712.1. NC_010682.1.

Genome annotation databases

EnsemblBacteriaiACD25280; ACD25280; Rpic_0117.
GeneIDi6288016.
KEGGirpi:Rpic_0117.
PATRICi20251096. VBIRalPic63053_1317.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001068 Genomic DNA. Translation: ACD25280.1 .
RefSeqi YP_001897712.1. NC_010682.1.

3D structure databases

ProteinModelPortali B2UDA1.
SMRi B2UDA1. Positions 26-330.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 402626.Rpic_0117.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD25280 ; ACD25280 ; Rpic_0117 .
GeneIDi 6288016.
KEGGi rpi:Rpic_0117.
PATRICi 20251096. VBIRalPic63053_1317.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci RPIC402626:GH94-116-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 12J.

Entry informationi

Entry nameiBIOB_RALPJ
AccessioniPrimary (citable) accession number: B2UDA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi