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B2UCX5 (DDL_RALPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Rpic_3087
OrganismRalstonia pickettii (strain 12J) [Complete proteome] [HAMAP]
Taxonomic identifier402626 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091206

Regions

Domain116 – 316201ATP-grasp
Nucleotide binding142 – 19756ATP By similarity

Sites

Metal binding2691Magnesium or manganese 1 By similarity
Metal binding2831Magnesium or manganese 1 By similarity
Metal binding2831Magnesium or manganese 2 By similarity
Metal binding2851Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B2UCX5 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: E1CBBA6043A361B9

FASTA33135,330
        10         20         30         40         50         60 
MTTGPFVPNP TIDPKSLGKV GVLMGGRSAE REISLMSGNG VLAALRARGV DAHAFDPGLQ 

        70         80         90        100        110        120 
AVADLAKQGF DRVVISLHGR FGEDGTIQGL LEQFGIPYTG SGVLASALAM DKEATKRQWQ 

       130        140        150        160        170        180 
THGLPTPDFV MLHAGADWQA VADRLGLPLI VKPAREGSSI GLTKVTSVAE LPAAYEKAAR 

       190        200        210        220        230        240 
LDRDVMAEQF IEGDELTCPI IGEGESATAL PLIRIVAPQA NYDYQNKYFT DDTRYECPAP 

       250        260        270        280        290        300 
IPADVAARVQ ALVVQAYRGL GCRGWGRADI MLRKSDNAPF LLEMNTSPGM TGHSLVPMGA 

       310        320        330 
RAAGISYEDF VLQLAASASL ELHASTDWKP E 

« Hide

References

[1]"Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 12J.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001068 Genomic DNA. Translation: ACD28210.1.
RefSeqYP_001900642.1. NC_010682.1.

3D structure databases

ProteinModelPortalB2UCX5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402626.Rpic_3087.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD28210; ACD28210; Rpic_3087.
GeneID6286114.
KEGGrpi:Rpic_3087.
PATRIC20256987. VBIRalPic63053_4225.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycRPIC402626:GH94-3127-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_RALPJ
AccessionPrimary (citable) accession number: B2UCX5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways