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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei639 – 6391ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRPIC402626:GH94-3022-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Rpic_2983
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000002566 Componenti: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 877877Leucine--tRNA ligasePRO_1000091349Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi402626.Rpic_2983.

Structurei

3D structure databases

ProteinModelPortaliB2UCA1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi636 – 6405"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2UCA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDKYSPSDV EQQAQQHWQA LDAYRVTEHA RAADGSDKPK FYACSMLPYP
60 70 80 90 100
SGKLHMGHVR NYTINDVMTR QLRMKGYNVL MPMGWDAFGM PAENAALNNG
110 120 130 140 150
VAPAAWTYDN IAYMKKQMQS MGLAIDWSRE VATCSPDYYR WNQWLFLKML
160 170 180 190 200
EKGIAYRKTG TVNWDPVDQT VLANEQVIDG RGWRSGAVVE KREIPMYYLG
210 220 230 240 250
ITKYAQELLS DLDPLGWPER VKLMQQNWIG KSEGVRFAFP HNISGDDGKL
260 270 280 290 300
INDGKLYVFT TRADTIMGVT FCAVAAEHPI ATHAAQSNPA LAAFIEECKH
310 320 330 340 350
GSVMEADMAT MEKKGMPTGL TVTHPLTGES VPVWVGNYVL MTYGDGAVMG
360 370 380 390 400
VPAHDERDFA FANKYHLPIK QVIDVKGQSY DTTTWADWYG DKEHGVLFHS
410 420 430 440 450
GKYDGLNYQQ AVDAVAADLA AQGLGEKKTT WRLRDWGISR QRYWGTPIPL
460 470 480 490 500
IHCESCGVVP VPEQDLPVRL PEDLVPDGTG NPLAKDPRFL NCTCPSCGKP
510 520 530 540 550
ARRETDTMDT FIDSCWYYMR YTCPDGETMV DARNDYWMPM DQYIGGIEHA
560 570 580 590 600
ILHLLYARFW TKVMRDLGLV KFDEPFTNLL TQGMVLNETY YREDASGKKQ
610 620 630 640 650
WINPADVDVQ TDERGRPVGA TLKADGQPVV IGGVEKMSKS KNNGIDPQAL
660 670 680 690 700
IDQYGADTAR LFTMFAAPPE QQLEWNDAGV EGASRFLRRL WNFGVVHGDA
710 720 730 740 750
IRGGHGNGVV AGATDADRAL RRELYTVLKQ ANYDYERLQY NTVVSATMKM
760 770 780 790 800
LNALEGAKDA GADARREGLG LLLRVLYPVV PHITHVLWTE LGYAGAYGDL
810 820 830 840 850
LDAPWPQVDE GALVQSEIEL VLQVNGKVRG SIVVPADADR AAIEAIAAKD
860 870
EAVQKFAEGK PPKKIIVVPG RLVNVVA
Length:877
Mass (Da):97,691
Last modified:July 1, 2008 - v1
Checksum:iF3F20541DD6871B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA. Translation: ACD28106.1.
RefSeqiYP_001900538.1. NC_010682.1.

Genome annotation databases

EnsemblBacteriaiACD28106; ACD28106; Rpic_2983.
KEGGirpi:Rpic_2983.
PATRICi20256773. VBIRalPic63053_4119.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA. Translation: ACD28106.1.
RefSeqiYP_001900538.1. NC_010682.1.

3D structure databases

ProteinModelPortaliB2UCA1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi402626.Rpic_2983.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD28106; ACD28106; Rpic_2983.
KEGGirpi:Rpic_2983.
PATRICi20256773. VBIRalPic63053_4119.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciRPIC402626:GH94-3022-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 12J.

Entry informationi

Entry nameiSYL_RALPJ
AccessioniPrimary (citable) accession number: B2UCA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: April 1, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.