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B2UBL7

- NAPA_RALPJ

UniProt

B2UBL7 - NAPA_RALPJ

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciRPIC402626:GH94-2883-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Rpic_2844
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000002566: Chromosome 1

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
BLAST
Chaini30 – 838809Periplasmic nitrate reductasePRO_5000369510Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi402626.Rpic_2844.

Structurei

3D structure databases

ProteinModelPortaliB2UBL7.
SMRiB2UBL7. Positions 41-835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2UBL7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVSRRAFIK QTAAAATASV AGVTLPAGAA NLVTDKELTQ LKWSKAPCRF
60 70 80 90 100
CGTGCGVEVA VKDNRVVATQ GDPKAEVNRG LNCVKGYFLS KIMYGKDRLT
110 120 130 140 150
QPLLRMKNGK YDKNGEFTPV TWDRAFDEMA MQFKRVIKEK GPTAVGMFGS
160 170 180 190 200
GQWTVFEGYA AVKLMKAGFR SNNLDPNARH CMASAVAGFM RTFGMDEPMG
210 220 230 240 250
CYDDFEVADA FVLWGSNMAE MHPILWSRVT DRRLSAPKTK VAVLSTFTHR
260 270 280 290 300
SFDLADIPIV FTPQADLAML NYIANYIITN KKVNTDFVNK HTVFKQGVTD
310 320 330 340 350
IGYGLRPDNP VQKAAKNADK VGDSKPISFD EFAKFVSTYD LDYTTKLANP
360 370 380 390 400
DKSKEKEFKQ QLQKLAELYA DPKIKVVSFW TMGFNQHTRG TWANNMIYNL
410 420 430 440 450
HLLTGKIATP GNSPFSLTGQ PSACGTAREV GTFSHRLPAD MVVTNPKHRE
460 470 480 490 500
EAEHIWKLPA GTIPEKPGYH AVLQNRMLKD GKLNAYWVQV NNNMQAGANI
510 520 530 540 550
NEEGYPGYRN PANFIVVSDV YPTVTALAAD LILPSAMWVE KEGAYGNAER
560 570 580 590 600
RTQFWHQLVD APGEARSDLW QLVEFSKRFK VEEVWPEDLI AKKPELRGKT
610 620 630 640 650
LYDVLYRNGN VDRFPLKDVD PEYNNAEAKA FGFYLQKGLF EEYASFGRGH
660 670 680 690 700
GHDLAPFDDY HKARGLRWPV VNGKETRWRY KEGSDPYVKA GTGYQFYGNP
710 720 730 740 750
DGKAVIFALP YEAPPEVPDK EYPFWLSTGR VLEHWHSGSM TRRVPELYRA
760 770 780 790 800
FPNAVCFMHP EDANALGLRR GVEVEVISRR GKMRTRIETR GRNQPPKGLV
810 820 830
FVPWFDASQL INKVTLDATC PISLQTDFKK CAVKIVKV
Length:838
Mass (Da):94,211
Last modified:July 1, 2008 - v1
Checksum:i10A3CEFE244E2E7E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001068 Genomic DNA. Translation: ACD27967.1.
RefSeqiYP_001900399.1. NC_010682.1.

Genome annotation databases

EnsemblBacteriaiACD27967; ACD27967; Rpic_2844.
GeneIDi6289539.
KEGGirpi:Rpic_2844.
PATRICi20256499. VBIRalPic63053_3982.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001068 Genomic DNA. Translation: ACD27967.1 .
RefSeqi YP_001900399.1. NC_010682.1.

3D structure databases

ProteinModelPortali B2UBL7.
SMRi B2UBL7. Positions 41-835.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 402626.Rpic_2844.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD27967 ; ACD27967 ; Rpic_2844 .
GeneIDi 6289539.
KEGGi rpi:Rpic_2844.
PATRICi 20256499. VBIRalPic63053_3982.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci RPIC402626:GH94-2883-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 12J.

Entry informationi

Entry nameiNAPA_RALPJ
AccessioniPrimary (citable) accession number: B2UBL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3