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B2UBL7 (NAPA_RALPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Rpic_2844
OrganismRalstonia pickettii (strain 12J) [Complete proteome] [HAMAP]
Taxonomic identifier402626 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Protein attributes

Sequence length838 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 838809Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_5000369510

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B2UBL7 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 10A3CEFE244E2E7E

FASTA83894,211
        10         20         30         40         50         60 
MKVSRRAFIK QTAAAATASV AGVTLPAGAA NLVTDKELTQ LKWSKAPCRF CGTGCGVEVA 

        70         80         90        100        110        120 
VKDNRVVATQ GDPKAEVNRG LNCVKGYFLS KIMYGKDRLT QPLLRMKNGK YDKNGEFTPV 

       130        140        150        160        170        180 
TWDRAFDEMA MQFKRVIKEK GPTAVGMFGS GQWTVFEGYA AVKLMKAGFR SNNLDPNARH 

       190        200        210        220        230        240 
CMASAVAGFM RTFGMDEPMG CYDDFEVADA FVLWGSNMAE MHPILWSRVT DRRLSAPKTK 

       250        260        270        280        290        300 
VAVLSTFTHR SFDLADIPIV FTPQADLAML NYIANYIITN KKVNTDFVNK HTVFKQGVTD 

       310        320        330        340        350        360 
IGYGLRPDNP VQKAAKNADK VGDSKPISFD EFAKFVSTYD LDYTTKLANP DKSKEKEFKQ 

       370        380        390        400        410        420 
QLQKLAELYA DPKIKVVSFW TMGFNQHTRG TWANNMIYNL HLLTGKIATP GNSPFSLTGQ 

       430        440        450        460        470        480 
PSACGTAREV GTFSHRLPAD MVVTNPKHRE EAEHIWKLPA GTIPEKPGYH AVLQNRMLKD 

       490        500        510        520        530        540 
GKLNAYWVQV NNNMQAGANI NEEGYPGYRN PANFIVVSDV YPTVTALAAD LILPSAMWVE 

       550        560        570        580        590        600 
KEGAYGNAER RTQFWHQLVD APGEARSDLW QLVEFSKRFK VEEVWPEDLI AKKPELRGKT 

       610        620        630        640        650        660 
LYDVLYRNGN VDRFPLKDVD PEYNNAEAKA FGFYLQKGLF EEYASFGRGH GHDLAPFDDY 

       670        680        690        700        710        720 
HKARGLRWPV VNGKETRWRY KEGSDPYVKA GTGYQFYGNP DGKAVIFALP YEAPPEVPDK 

       730        740        750        760        770        780 
EYPFWLSTGR VLEHWHSGSM TRRVPELYRA FPNAVCFMHP EDANALGLRR GVEVEVISRR 

       790        800        810        820        830 
GKMRTRIETR GRNQPPKGLV FVPWFDASQL INKVTLDATC PISLQTDFKK CAVKIVKV 

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References

[1]"Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 12J.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001068 Genomic DNA. Translation: ACD27967.1.
RefSeqYP_001900399.1. NC_010682.1.

3D structure databases

ProteinModelPortalB2UBL7.
SMRB2UBL7. Positions 41-835.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402626.Rpic_2844.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD27967; ACD27967; Rpic_2844.
GeneID6289539.
KEGGrpi:Rpic_2844.
PATRIC20256499. VBIRalPic63053_3982.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAHPKTRVA.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycRPIC402626:GH94-2883-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_RALPJ
AccessionPrimary (citable) accession number: B2UBL7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families