Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei85Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei152Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei181Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei382Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei386Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei492Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei541Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei568Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei804Substrate; via amide nitrogenUniRule annotation1
Binding sitei812Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei829Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Rpic_2844
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000002566 Componenti: Chromosome 1

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Tat-type signalUniRule annotationAdd BLAST29
ChainiPRO_500036951030 – 838Periplasmic nitrate reductaseUniRule annotationAdd BLAST809

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Proteomic databases

PRIDEiB2UBL7

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi402626.Rpic_2844

Structurei

3D structure databases

ProteinModelPortaliB2UBL7
SMRiB2UBL7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 974Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 221Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni245 – 249Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni518 – 519Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni728 – 737Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2UBL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSRRAFIK QTAAAATASV AGVTLPAGAA NLVTDKELTQ LKWSKAPCRF
60 70 80 90 100
CGTGCGVEVA VKDNRVVATQ GDPKAEVNRG LNCVKGYFLS KIMYGKDRLT
110 120 130 140 150
QPLLRMKNGK YDKNGEFTPV TWDRAFDEMA MQFKRVIKEK GPTAVGMFGS
160 170 180 190 200
GQWTVFEGYA AVKLMKAGFR SNNLDPNARH CMASAVAGFM RTFGMDEPMG
210 220 230 240 250
CYDDFEVADA FVLWGSNMAE MHPILWSRVT DRRLSAPKTK VAVLSTFTHR
260 270 280 290 300
SFDLADIPIV FTPQADLAML NYIANYIITN KKVNTDFVNK HTVFKQGVTD
310 320 330 340 350
IGYGLRPDNP VQKAAKNADK VGDSKPISFD EFAKFVSTYD LDYTTKLANP
360 370 380 390 400
DKSKEKEFKQ QLQKLAELYA DPKIKVVSFW TMGFNQHTRG TWANNMIYNL
410 420 430 440 450
HLLTGKIATP GNSPFSLTGQ PSACGTAREV GTFSHRLPAD MVVTNPKHRE
460 470 480 490 500
EAEHIWKLPA GTIPEKPGYH AVLQNRMLKD GKLNAYWVQV NNNMQAGANI
510 520 530 540 550
NEEGYPGYRN PANFIVVSDV YPTVTALAAD LILPSAMWVE KEGAYGNAER
560 570 580 590 600
RTQFWHQLVD APGEARSDLW QLVEFSKRFK VEEVWPEDLI AKKPELRGKT
610 620 630 640 650
LYDVLYRNGN VDRFPLKDVD PEYNNAEAKA FGFYLQKGLF EEYASFGRGH
660 670 680 690 700
GHDLAPFDDY HKARGLRWPV VNGKETRWRY KEGSDPYVKA GTGYQFYGNP
710 720 730 740 750
DGKAVIFALP YEAPPEVPDK EYPFWLSTGR VLEHWHSGSM TRRVPELYRA
760 770 780 790 800
FPNAVCFMHP EDANALGLRR GVEVEVISRR GKMRTRIETR GRNQPPKGLV
810 820 830
FVPWFDASQL INKVTLDATC PISLQTDFKK CAVKIVKV
Length:838
Mass (Da):94,211
Last modified:July 1, 2008 - v1
Checksum:i10A3CEFE244E2E7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA Translation: ACD27967.1
RefSeqiWP_012436328.1, NC_010682.1

Genome annotation databases

EnsemblBacteriaiACD27967; ACD27967; Rpic_2844
GeneIDi6289539
KEGGirpi:Rpic_2844
PATRICifig|402626.5.peg.3982

Similar proteinsi

Entry informationi

Entry nameiNAPA_RALPJ
AccessioniPrimary (citable) accession number: B2UBL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health