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B2UAQ4

- SYI_RALPJ

UniProt

B2UAQ4 - SYI_RALPJ

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei592 – 5921Aminoacyl-adenylateUniRule annotation
Binding sitei636 – 6361ATPUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi942 – 9421ZincUniRule annotation
Metal bindingi945 – 9451ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRPIC402626:GH94-2765-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Rpic_2726
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000002566: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959Isoleucine--tRNA ligasePRO_1000189190Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi402626.Rpic_2726.

Structurei

3D structure databases

ProteinModelPortaliB2UAQ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionAdd
BLAST
Motifi633 – 6375"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2UAQ4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDSKKPTPE KSKYPVNLLD TPFPMRGDLP KREPLWVKQW QDKQLYKKIR
60 70 80 90 100
AARKGAKKFI LHDGPPYANG DLHIGHAVNK ILKDMVIKAR GLTGLDAVYV
110 120 130 140 150
PGWDCHGMPI EIQIEKQFGK GLPVKEVQEK ARAYATGQIA RQKADFERLG
160 170 180 190 200
VLGDWADPYL TMNFRNEADE VRALGKILEK GYVFRGLKPV NWCFDCGSAL
210 220 230 240 250
AEAEVEYADR TDLSIDVGFP FADIDALASA FHVGADVLKA KPGWIVIWTT
260 270 280 290 300
TPWTIPSNQA LNLHPEIEYA LVDTSRGLLI VAKERVEACL QSWKLEGTVL
310 320 330 340 350
ATCEGAALNG VRFHHPLAKM DAGYDRTSPV YLGDYVTIDT GTGIVHSAPA
360 370 380 390 400
YGVEDFQSCK AHNMPDSEII NPVMGNGVYA STLPLFGGQM IWDANPKIVE
410 420 430 440 450
VLRESGNLFD AHKYAHSYMH CWRHKTPIIY RATSQWFAGM DVQPNDGNAT
460 470 480 490 500
LRETALAGID ATAFYPSWGK QRLHNMIANR PDWTLSRQRQ WGVPMAFFVH
510 520 530 540 550
KETGALHPRT PELLEQVAQR IEKSGIEAWQ TLDPRELLGD EADMYEKNRD
560 570 580 590 600
TLDVWFDSGT THWHVIRGSH AADLYDASAD LPDGRLADLY LEGSDQHRGW
610 620 630 640 650
FHSSLLTASM LYGKPPYKGL LTHGFTVDGE GRKMSKSIGN TIAPQEIANK
660 670 680 690 700
MGAEIIRLWV ASTDYSGELA ISDEILKRVV EGYRRIRNTL RFLLANLTDY
710 720 730 740 750
DHAKHALPTE QWLEIDRYAV ALTDALQKEV LSHYDVYEFH PVVAKLQTFC
760 770 780 790 800
SEDLGGFYLD VLKDRLYTTA PDSVARRSAQ NALYHITQAM LHWMAPFLSF
810 820 830 840 850
TAEEAWQVFA HGTEHTDTIF TSTYYNAPVP QGASALLEKW AAIRNVRGEV
860 870 880 890 900
TKQLEALRID GKIGSSLQAE VTVSAGESVH DALASLGDDL RFVLITSAAK
910 920 930 940 950
LERAPEGGDL LITVVPSTHS KCERCWHYRA DVGHDHAHPT LCKRCTDNLF

GSGEQRSAA
Length:959
Mass (Da):107,152
Last modified:July 1, 2008 - v1
Checksum:i3DFB8985DDEA1D2A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001068 Genomic DNA. Translation: ACD27851.1.
RefSeqiYP_001900283.1. NC_010682.1.

Genome annotation databases

EnsemblBacteriaiACD27851; ACD27851; Rpic_2726.
GeneIDi6288807.
KEGGirpi:Rpic_2726.
PATRICi20256258. VBIRalPic63053_3862.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001068 Genomic DNA. Translation: ACD27851.1 .
RefSeqi YP_001900283.1. NC_010682.1.

3D structure databases

ProteinModelPortali B2UAQ4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 402626.Rpic_2726.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD27851 ; ACD27851 ; Rpic_2726 .
GeneIDi 6288807.
KEGGi rpi:Rpic_2726.
PATRICi 20256258. VBIRalPic63053_3862.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RPIC402626:GH94-2765-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 12J.

Entry informationi

Entry nameiSYI_RALPJ
AccessioniPrimary (citable) accession number: B2UAQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3