B2U968 (FABH_RALPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.180

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	Rpic_0915

Organism

Ralstonia pickettii (strain 12J) [Complete proteome] [HAMAP]

Taxonomic identifier

402626 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm By similarity HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000187890
Regions
	Region	254 – 258	5	ACP-binding By similarity
Sites
	Active site	120	1	By similarity
	Active site	253	1	By similarity
	Active site	283	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2U968 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 585152A28CE54B5E	FASTA	326	34,516
	10 20 30 40 50 60 MTRFARIIGT GSYLPPKRVT NDELAAQLAE KGIETSDEWI VSRSGIRARH FAEPDVTCSD 70 80 90 100 110 120 LAVRAAERAI EAAGIDRSEL DLILVATSTP DFVFPSTACL VQQKLGITNG CAAFDVQAVC 130 140 150 160 170 180 SGFVYALATA DKFIRSGAYR NVLVIGSEVF SRIIDFSDRT TCVLFGDGAG AVVLQASDEP 190 200 210 220 230 240 GILSTALHAD GSHANILCVP GNVAAGAIQG SAFLYMDGQA VFKLAVTVLD KVAREALAAA 250 260 270 280 290 300 ELDASQVDWL IPHQANIRIM QGTTKKLGLS NERLIVTVDE HGNTSAASIP LALDVAVRDG 310 320 RIQRGQHVML EGVGGGFTWG AALLRF

References

[1]

"Complete sequence of chromosome 1 of Ralstonia pickettii 12J." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marsh T., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12J.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001068 Genomic DNA. Translation: ACD26065.1.
RefSeq	YP_001898497.1. NC_010682.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2U968.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6288548.
GenomeReviews	Gene locus Rpic_0915 in contig CP001068_GR.
KEGG	rpi:Rpic_0915.
PATRIC	20252702. VBIRalPic63053_2115.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649927.
OMA	RILNFLA.
ProtClustDB	PRK09352.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_RALPJ

Accession

Primary (citable) accession number: B2U968

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program