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Protein

Uracil phosphoribosyltransferase

Gene

upp

Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.UniRule annotation

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP.UniRule annotation

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uracil.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uracil phosphoribosyltransferase (upp)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uracil, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 8515-phospho-alpha-D-ribose 1-diphosphateUniRule annotation
Binding sitei110 – 11015-phospho-alpha-D-ribose 1-diphosphateUniRule annotation
Binding sitei200 – 2001Uracil; via amide nitrogenUniRule annotation
Binding sitei206 – 20615-phospho-alpha-D-ribose 1-diphosphateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciRPIC402626:GH94-2422-MONOMER.
UniPathwayiUPA00574; UER00636.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil phosphoribosyltransferaseUniRule annotation (EC:2.4.2.9UniRule annotation)
Alternative name(s):
UMP pyrophosphorylaseUniRule annotation
UPRTaseUniRule annotation
Gene namesi
Name:uppUniRule annotation
Ordered Locus Names:Rpic_2386
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000002566 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Uracil phosphoribosyltransferasePRO_1000139150Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi402626.Rpic_2386.

Structurei

3D structure databases

ProteinModelPortaliB2U8Z0.
SMRiB2U8Z0. Positions 9-215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 14395-phospho-alpha-D-ribose 1-diphosphate bindingUniRule annotation
Regioni205 – 2073Uracil bindingUniRule annotation

Sequence similaritiesi

Belongs to the UPRTase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ5. Bacteria.
COG0035. LUCA.
HOGENOMiHOG000262754.
KOiK00761.
OMAiHKLSHMR.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_B. Upp_B. 1 hit.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.

Sequencei

Sequence statusi: Complete.

B2U8Z0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQDPRFPNL SILNHPLIQH KLTHMRDKDT STRTFRELLR EITLLMGYEI
60 70 80 90 100
TRNLPLTSRH IDTPMGPMEA PVIAGRKLAV VPVLRAGVGM SDGLVELIPS
110 120 130 140 150
ARIGHIGVYR DDQHRPVEYL VRLPDLEDRT FILCDPMVAT GYSAVHAVDV
160 170 180 190 200
MKKRGVPDEN ILFLALVAAP EGVEVFQKAH PGVKLFVASL DSHLDENAYI
210
IPGLGDAGDR LFGTKN
Length:216
Mass (Da):24,001
Last modified:July 1, 2008 - v1
Checksum:i3D349733B9B8BBA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA. Translation: ACD27520.1.
RefSeqiWP_009241133.1. NC_010682.1.

Genome annotation databases

EnsemblBacteriaiACD27520; ACD27520; Rpic_2386.
GeneIDi6288543.
KEGGirpi:Rpic_2386.
PATRICi20255572. VBIRalPic63053_3522.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA. Translation: ACD27520.1.
RefSeqiWP_009241133.1. NC_010682.1.

3D structure databases

ProteinModelPortaliB2U8Z0.
SMRiB2U8Z0. Positions 9-215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi402626.Rpic_2386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD27520; ACD27520; Rpic_2386.
GeneIDi6288543.
KEGGirpi:Rpic_2386.
PATRICi20255572. VBIRalPic63053_3522.

Phylogenomic databases

eggNOGiENOG4105CZ5. Bacteria.
COG0035. LUCA.
HOGENOMiHOG000262754.
KOiK00761.
OMAiHKLSHMR.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.
BioCyciRPIC402626:GH94-2422-MONOMER.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_B. Upp_B. 1 hit.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUPP_RALPJ
AccessioniPrimary (citable) accession number: B2U8Z0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: September 7, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.