B2U8M4 (B2U8M4_RALPJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Thymidylate synthase HAMAP MF_00008
Short name=TS HAMAP MF_00008
Short name=TSase HAMAP MF_00008
EC=2.1.1.45 HAMAP MF_00008

Gene names

Name:	thyA HAMAP MF_00008
Ordered Locus Names:	Rpic_0818

Organism

Ralstonia pickettii (strain 12J) [Complete proteome] [HAMAP]

Taxonomic identifier

402626 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451
Subunit structure	Homodimer By similarity. HAMAP MF_00008 SAAS SAAS023451
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. RuleBase RU004150 Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. HAMAP MF_00008

Ontologies

Keywords
Biological process	Nucleotide biosynthesis HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451
Cellular component	Cytoplasm HAMAP MF_00008
Molecular function	Methyltransferase HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451 EMBL ACD25968.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	146	1	By similarity HAMAP MF_00008

Sequences

	Sequence		Length	Mass (Da)
	B2U8M4 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 5228878BDC39D475	FASTA	264	30,276
	10 20 30 40 50 60 MKPYLDLMRH VYEHGTDKAD RTGTGTRSVF GHQMRFNLQD GFPLVTTKKV HIKSIVYELL 70 80 90 100 110 120 WFLQGSTNVR WLQDNGVTIW DEWADADGEL GPVYGSQWRS WPTPNGEHID QIAELIAQIK 130 140 150 160 170 180 RNPDSRRLIV SAWNVADIPR MKLPPCHAFF QFYVADGKLS CQLYQRSADI FLGVPFNIAS 190 200 210 220 230 240 YALLVHMVAQ QTGLDVGDFV WTGGDCHLYN NHFEQVQTQL AREPLPLPKL IIKRKPESIF 250 260 DYQYEDFEIL GYQSHPAIKA PVAV

References

[1]

"Complete sequence of chromosome 1 of Ralstonia pickettii 12J." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marsh T., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001068 Genomic DNA. Translation: ACD25968.1.
RefSeq	YP_001898400.1. NC_010682.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2U8M4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6286502.
GenomeReviews	Gene locus Rpic_0818 in contig CP001068_GR.
KEGG	rpi:Rpic_0818.
PATRIC	20252500. VBIRalPic63053_2015.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG588098.
OMA	DIPRMKL.
ProtClustDB	PRK01827.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B2U8M4_RALPJ

Accession

Primary (citable) accession number: B2U8M4

Entry history

Integrated into UniProtKB/TrEMBL:	July 1, 2008
Last sequence update:	July 1, 2008
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)