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B2U846 (SYQ_RALPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:Rpic_0737
OrganismRalstonia pickettii (strain 12J) [Complete proteome] [HAMAP]
Taxonomic identifier402626 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_1000095507

Regions

Motif47 – 5711"HIGH" region HAMAP-Rule MF_00126
Motif290 – 2945"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2931ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2U846 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 4BA4948AED3B03E4

FASTA57665,202
        10         20         30         40         50         60 
MSQDNATAPT SNFLRQIIDA DLAQGTYADR KDATGQPIPP VVTRFPPEPN GYLHIGHAKS 

        70         80         90        100        110        120 
IWVNFGMARD YNGRCHLRFD DTNPVKEDTE YVDSIIDAVH WLGYSWSDAG GEHLYYASDY 

       130        140        150        160        170        180 
FEQLYGFAEV LIQRGVAYVD SQSAEQIAAN RGDFTRPGTL SPFRDRSVDE NLALFRDMRA 

       190        200        210        220        230        240 
GKYKDGEHVL RAKIDMAAPN IVMRDPVLYR IRHAHHHRTG DAWCIYPMYD FTHCISDALE 

       250        260        270        280        290        300 
NITHSLCTLE FENNRPLYDW VLDHLRDAGV LAAPLPHQYE FARLHLTYAI TSKRKLLQLV 

       310        320        330        340        350        360 
TEKRVDGWDD PRMPTLVGIR RRGYTPESIQ LFCERVGVSK ADSWIDMSIL EGAVRDDLDA 

       370        380        390        400        410        420 
RAPRSVAVLD PVKLVLDNVP ADFNEPCSAP VHPKQPELGR REFPLTRELW IEREDFTETP 

       430        440        450        460        470        480 
PKGYFRLFPG NKVRLRYGYV IECTGCDKDA AGNITAVHAN IIPDTKSGTP GADSVKVKGN 

       490        500        510        520        530        540 
IHWVSAAHAL EAEVRLYDRL FSDPQPDSGD KNFLDALNPN SKKIVKAFLE PTLATAKAED 

       550        560        570 
RFQFERHGYF VADRIDSQPG KPVFNRVVGL KDSWGK 

« Hide

References

[1]"Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 12J.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001068 Genomic DNA. Translation: ACD25888.1.
RefSeqYP_001898320.1. NC_010682.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402626.Rpic_0737.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD25888; ACD25888; Rpic_0737.
GeneID6286713.
KEGGrpi:Rpic_0737.
PATRIC20252340. VBIRalPic63053_1935.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMAVTHSICT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK05347.

Enzyme and pathway databases

BioCycRPIC402626:GH94-741-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_RALPJ
AccessionPrimary (citable) accession number: B2U846
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries