Reviewed, UniProtKB/Swiss-Prot B2U846 (SYQ_RALPJ)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	Rpic_0737

Organism

Ralstonia pickettii (strain 12J) [Complete proteome] [HAMAP]

Taxonomic identifier

402626 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_1000095507
Regions
	Motif	47 – 57	11	"HIGH" region HAMAP MF_00126
	Motif	290 – 294	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	293	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2U846-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 4BA4948AED3B03E4	FASTA	576	65,202
	10 20 30 40 50 60 MSQDNATAPT SNFLRQIIDA DLAQGTYADR KDATGQPIPP VVTRFPPEPN GYLHIGHAKS 70 80 90 100 110 120 IWVNFGMARD YNGRCHLRFD DTNPVKEDTE YVDSIIDAVH WLGYSWSDAG GEHLYYASDY 130 140 150 160 170 180 FEQLYGFAEV LIQRGVAYVD SQSAEQIAAN RGDFTRPGTL SPFRDRSVDE NLALFRDMRA 190 200 210 220 230 240 GKYKDGEHVL RAKIDMAAPN IVMRDPVLYR IRHAHHHRTG DAWCIYPMYD FTHCISDALE 250 260 270 280 290 300 NITHSLCTLE FENNRPLYDW VLDHLRDAGV LAAPLPHQYE FARLHLTYAI TSKRKLLQLV 310 320 330 340 350 360 TEKRVDGWDD PRMPTLVGIR RRGYTPESIQ LFCERVGVSK ADSWIDMSIL EGAVRDDLDA 370 380 390 400 410 420 RAPRSVAVLD PVKLVLDNVP ADFNEPCSAP VHPKQPELGR REFPLTRELW IEREDFTETP 430 440 450 460 470 480 PKGYFRLFPG NKVRLRYGYV IECTGCDKDA AGNITAVHAN IIPDTKSGTP GADSVKVKGN 490 500 510 520 530 540 IHWVSAAHAL EAEVRLYDRL FSDPQPDSGD KNFLDALNPN SKKIVKAFLE PTLATAKAED 550 560 570 RFQFERHGYF VADRIDSQPG KPVFNRVVGL KDSWGK

References

[1]

"Complete sequence of chromosome 1 of Ralstonia pickettii 12J." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marsh T., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001068 Genomic DNA. Translation: ACD25888.1.
RefSeq	YP_001898320.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6286713.
GenomeReviews	Gene locus Rpic_0737 in contig CP001068_GR.
KEGG	rpi:Rpic_0737.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NFLRQII.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_RALPJ

Accession

Primary (citable) accession number: B2U846

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)