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B2U804

- PCKG_RALPJ

UniProt

B2U804 - PCKG_RALPJ

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
pckG, Rpic_3739
Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851Substrate By similarity
Binding sitei226 – 2261Substrate; via amide nitrogen By similarity
Metal bindingi233 – 2331Manganese By similarity
Binding sitei233 – 2331Substrate By similarity
Metal bindingi253 – 2531Manganese; via tele nitrogen By similarity
Binding sitei275 – 2751Substrate By similarity
Active sitei277 – 2771 By similarity
Metal bindingi302 – 3021Manganese By similarity
Binding sitei402 – 4021GTP By similarity
Binding sitei433 – 4331GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2816GTP By similarity
Nucleotide bindingi530 – 5334GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRPIC402626:GH94-3797-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Ordered Locus Names:Rpic_3739
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000002566: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation
PRO_1000125047Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi402626.Rpic_3739.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni400 – 4023Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWDNPAGV.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2U804-1 [UniParc]FASTAAdd to Basket

« Hide

MNQPVMQGVP ALNVPDYVKH PRLVAWVGEV AALTKPERIV WCDGSQEEYD    50
RLCAEMVAAG TMKQLNPAKR KNSYLALSDP SDVARVEDRT FICSQKKEDA 100
GPTNNWTAPA EMRQTLNGLF DGSMRGRTLY VVPFSMGPLG SPIAHIGVEL 150
SDSPYVAVNM RIMTRMGRAV YDVLGTDGDF VPCVHTVGKP LAAGEKDVPW 200
PCNPTKYIVH FPETREIWSF GSGYGGNALL GKKCFALRIA STMGRDQGWL 250
AEHMLILGVT SPEGKTYHVA AAFPSACGKT NFAMLIPPAG LNGWKVTTIG 300
DDIAWIKPRQ DANGKTRLYA INPEAGYFGV APGTSEKTNF NAMATLKENV 350
IFTNVALTDD GDVWWEGMTD TPPAHLIDWQ GQDWTPASAK ETGRKAAHPN 400
ARFTAPAAQC PSIDPEWDNP AGVPIDAFIF GGRRSTTVPL VTEARDWTEG 450
VYMAATMGSE TTAAAAGQQG VVRRDPFAML PFCGYNMADY FAHWLKVGEK 500
LADSGATLPK IFCVNWFRKD ENGKFVWPGF GENMRVLKWM IDRIEGQAQG 550
EEHVFGVSPR YEELSWDGLD FTAEQFAKVI SLDADAWKQE LALHDELFTK 600
LAHGLPQALP DAKTRLEERL GG 622
Length:622
Mass (Da):68,301
Last modified:July 1, 2008 - v1
Checksum:i32728841312A061E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001068 Genomic DNA. Translation: ACD28857.1.
RefSeqiYP_001901289.1. NC_010682.1.

Genome annotation databases

EnsemblBacteriaiACD28857; ACD28857; Rpic_3739.
GeneIDi6286862.
KEGGirpi:Rpic_3739.
PATRICi20258327. VBIRalPic63053_4877.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001068 Genomic DNA. Translation: ACD28857.1 .
RefSeqi YP_001901289.1. NC_010682.1.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 402626.Rpic_3739.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD28857 ; ACD28857 ; Rpic_3739 .
GeneIDi 6286862.
KEGGi rpi:Rpic_3739.
PATRICi 20258327. VBIRalPic63053_4877.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi WDNPAGV.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci RPIC402626:GH94-3797-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 12J.

Entry informationi

Entry nameiPCKG_RALPJ
AccessioniPrimary (citable) accession number: B2U804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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