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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85SubstrateUniRule annotation1
Metal bindingi233ManganeseUniRule annotation1
Metal bindingi253Manganese; via tele nitrogenUniRule annotation1
Binding sitei275SubstrateUniRule annotation1
Active sitei277UniRule annotation1
Metal bindingi302ManganeseUniRule annotation1
Binding sitei402GTPUniRule annotation1
Binding sitei433GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi276 – 281GTPUniRule annotation6
Nucleotide bindingi530 – 533GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Rpic_3739
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000002566 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001250471 – 622Phosphoenolpyruvate carboxykinase [GTP]Add BLAST622

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi402626.Rpic_3739.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni224 – 226Substrate bindingUniRule annotation3
Regioni400 – 402Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2U804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQPVMQGVP ALNVPDYVKH PRLVAWVGEV AALTKPERIV WCDGSQEEYD
60 70 80 90 100
RLCAEMVAAG TMKQLNPAKR KNSYLALSDP SDVARVEDRT FICSQKKEDA
110 120 130 140 150
GPTNNWTAPA EMRQTLNGLF DGSMRGRTLY VVPFSMGPLG SPIAHIGVEL
160 170 180 190 200
SDSPYVAVNM RIMTRMGRAV YDVLGTDGDF VPCVHTVGKP LAAGEKDVPW
210 220 230 240 250
PCNPTKYIVH FPETREIWSF GSGYGGNALL GKKCFALRIA STMGRDQGWL
260 270 280 290 300
AEHMLILGVT SPEGKTYHVA AAFPSACGKT NFAMLIPPAG LNGWKVTTIG
310 320 330 340 350
DDIAWIKPRQ DANGKTRLYA INPEAGYFGV APGTSEKTNF NAMATLKENV
360 370 380 390 400
IFTNVALTDD GDVWWEGMTD TPPAHLIDWQ GQDWTPASAK ETGRKAAHPN
410 420 430 440 450
ARFTAPAAQC PSIDPEWDNP AGVPIDAFIF GGRRSTTVPL VTEARDWTEG
460 470 480 490 500
VYMAATMGSE TTAAAAGQQG VVRRDPFAML PFCGYNMADY FAHWLKVGEK
510 520 530 540 550
LADSGATLPK IFCVNWFRKD ENGKFVWPGF GENMRVLKWM IDRIEGQAQG
560 570 580 590 600
EEHVFGVSPR YEELSWDGLD FTAEQFAKVI SLDADAWKQE LALHDELFTK
610 620
LAHGLPQALP DAKTRLEERL GG
Length:622
Mass (Da):68,301
Last modified:July 1, 2008 - v1
Checksum:i32728841312A061E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA. Translation: ACD28857.1.
RefSeqiWP_009239362.1. NC_010682.1.

Genome annotation databases

EnsemblBacteriaiACD28857; ACD28857; Rpic_3739.
GeneIDi6286862.
KEGGirpi:Rpic_3739.
PATRICi20258327. VBIRalPic63053_4877.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA. Translation: ACD28857.1.
RefSeqiWP_009239362.1. NC_010682.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi402626.Rpic_3739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD28857; ACD28857; Rpic_3739.
GeneIDi6286862.
KEGGirpi:Rpic_3739.
PATRICi20258327. VBIRalPic63053_4877.

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_RALPJ
AccessioniPrimary (citable) accession number: B2U804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: November 2, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.