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B2U804

- PCKG_RALPJ

UniProt

B2U804 - PCKG_RALPJ

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn(2+) ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851SubstrateUniRule annotation
Binding sitei226 – 2261Substrate; via amide nitrogenUniRule annotation
Metal bindingi233 – 2331ManganeseUniRule annotation
Binding sitei233 – 2331SubstrateUniRule annotation
Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
Binding sitei275 – 2751SubstrateUniRule annotation
Active sitei277 – 2771UniRule annotation
Metal bindingi302 – 3021ManganeseUniRule annotation
Binding sitei402 – 4021GTPUniRule annotation
Binding sitei433 – 4331GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2816GTPUniRule annotation
Nucleotide bindingi530 – 5334GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRPIC402626:GH94-3797-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Rpic_3739
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000002566: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Phosphoenolpyruvate carboxykinase [GTP]PRO_1000125047Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi402626.Rpic_3739.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni400 – 4023Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWDNPAGV.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2U804-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNQPVMQGVP ALNVPDYVKH PRLVAWVGEV AALTKPERIV WCDGSQEEYD
60 70 80 90 100
RLCAEMVAAG TMKQLNPAKR KNSYLALSDP SDVARVEDRT FICSQKKEDA
110 120 130 140 150
GPTNNWTAPA EMRQTLNGLF DGSMRGRTLY VVPFSMGPLG SPIAHIGVEL
160 170 180 190 200
SDSPYVAVNM RIMTRMGRAV YDVLGTDGDF VPCVHTVGKP LAAGEKDVPW
210 220 230 240 250
PCNPTKYIVH FPETREIWSF GSGYGGNALL GKKCFALRIA STMGRDQGWL
260 270 280 290 300
AEHMLILGVT SPEGKTYHVA AAFPSACGKT NFAMLIPPAG LNGWKVTTIG
310 320 330 340 350
DDIAWIKPRQ DANGKTRLYA INPEAGYFGV APGTSEKTNF NAMATLKENV
360 370 380 390 400
IFTNVALTDD GDVWWEGMTD TPPAHLIDWQ GQDWTPASAK ETGRKAAHPN
410 420 430 440 450
ARFTAPAAQC PSIDPEWDNP AGVPIDAFIF GGRRSTTVPL VTEARDWTEG
460 470 480 490 500
VYMAATMGSE TTAAAAGQQG VVRRDPFAML PFCGYNMADY FAHWLKVGEK
510 520 530 540 550
LADSGATLPK IFCVNWFRKD ENGKFVWPGF GENMRVLKWM IDRIEGQAQG
560 570 580 590 600
EEHVFGVSPR YEELSWDGLD FTAEQFAKVI SLDADAWKQE LALHDELFTK
610 620
LAHGLPQALP DAKTRLEERL GG
Length:622
Mass (Da):68,301
Last modified:July 1, 2008 - v1
Checksum:i32728841312A061E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA. Translation: ACD28857.1.
RefSeqiYP_001901289.1. NC_010682.1.

Genome annotation databases

EnsemblBacteriaiACD28857; ACD28857; Rpic_3739.
GeneIDi6286862.
KEGGirpi:Rpic_3739.
PATRICi20258327. VBIRalPic63053_4877.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA. Translation: ACD28857.1 .
RefSeqi YP_001901289.1. NC_010682.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 402626.Rpic_3739.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD28857 ; ACD28857 ; Rpic_3739 .
GeneIDi 6286862.
KEGGi rpi:Rpic_3739.
PATRICi 20258327. VBIRalPic63053_4877.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi WDNPAGV.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci RPIC402626:GH94-3797-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 12J.

Entry informationi

Entry nameiPCKG_RALPJ
AccessioniPrimary (citable) accession number: B2U804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: November 26, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3