Reviewed, UniProtKB/Swiss-Prot B2U7J7 (T23O_RALPJ)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	Rpic_0708

Organism

Ralstonia pickettii (strain 12J) [Complete proteome] [HAMAP]

Taxonomic identifier

402626 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Tryptophan 2,3-dioxygenase		PRO_0000360129
Regions
	Region	22 – 26	5	Substrate binding By similarity
	Region	47 – 51	5	Substrate binding By similarity
Sites
	Metal binding	236	1	Iron (heme axial ligand) By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	113	1	Substrate By similarity
	Binding site	120	1	Heme By similarity
	Binding site	250	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2U7J7-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: A90A7E57A315870A	FASTA	278	32,492
	10 20 30 40 50 60 MQAAQGEGWH DAQLDFSKSM SYGDYLALDQ ILNAQHPRSP DHNEMLFIVQ HQTTELWMKL 70 80 90 100 110 120 MLHELRAARD CVRNDNLPPA FKMLARVSRI MDQLVQAWNV LATMTPPEYS AMRPHLGQSS 130 140 150 160 170 180 GFQSYQYREI EFILGNKNAA MLKPHAHRTE HYEQVKAALE TPSLYDEAVR YMARHGFAFD 190 200 210 220 230 240 ADCIERDWSR PVTYNASVEA AWLEVYRDPT HHWELYELAE KFVDLEDAFR QWRFRHVTTV 250 260 270 ERVIGFKRGT GGTEGVGYLR KMLDVVLFPE LWKLRTDL

References

[1]

"Complete sequence of chromosome 1 of Ralstonia pickettii 12J." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marsh T., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001068 Genomic DNA. Translation: ACD25859.1.
RefSeq	YP_001898291.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6288350.
GenomeReviews	Gene locus Rpic_0708 in contig CP001068_GR.
KEGG	rpi:Rpic_0708.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MKLILHE.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_RALPJ

Accession

Primary (citable) accession number: B2U7J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)