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B2U7E2

- TRMB_RALPJ

UniProt

B2U7E2 - TRMB_RALPJ

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei89 – 891S-adenosyl-L-methionineUniRule annotation
    Binding sitei114 – 1141S-adenosyl-L-methionineUniRule annotation
    Binding sitei141 – 1411S-adenosyl-L-methionineUniRule annotation
    Active sitei164 – 1641By similarity
    Binding sitei164 – 1641S-adenosyl-L-methionineUniRule annotation
    Binding sitei168 – 1681SubstrateUniRule annotation
    Binding sitei200 – 2001SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciRPIC402626:GH94-654-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:Rpic_0653
    OrganismiRalstonia pickettii (strain 12J)
    Taxonomic identifieri402626 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
    ProteomesiUP000002566: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 257257tRNA (guanine-N(7)-)-methyltransferasePRO_1000136360Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi402626.Rpic_0653.

    Structurei

    3D structure databases

    ProteinModelPortaliB2U7E2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni170 – 1756Interaction with RNAUniRule annotation
    Regioni235 – 2384Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiHSTLEMA.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2U7E2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQPIEQPGTG PDDITPESQD TNTAESAESG AETGHPRRIR SFVRRAGRTS    50
    TGQQRAINEL GPRFQLPYTT EPLDWDAAFG RAGAKRIFEI GFGMGETTAH 100
    IAQLRPDDDF LGVEVHEPGV GALLKLIGER EIGNIRIVSH DAVEVLAQMI 150
    PEGTLDGIHV FFPDPWHKKR HNKRRLIQSP FVARLAAHLK PGGYLHCATD 200
    WEEYAHQMLE VLSSEPTLEN TADGFAPRPD YRPVTKFEKR GLRLGHGVWD 250
    VVFRKRG 257
    Length:257
    Mass (Da):28,698
    Last modified:July 1, 2008 - v1
    Checksum:iCD5E175EE75708D6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001068 Genomic DNA. Translation: ACD25804.1.
    RefSeqiYP_001898236.1. NC_010682.1.

    Genome annotation databases

    EnsemblBacteriaiACD25804; ACD25804; Rpic_0653.
    GeneIDi6288040.
    KEGGirpi:Rpic_0653.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001068 Genomic DNA. Translation: ACD25804.1 .
    RefSeqi YP_001898236.1. NC_010682.1.

    3D structure databases

    ProteinModelPortali B2U7E2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 402626.Rpic_0653.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACD25804 ; ACD25804 ; Rpic_0653 .
    GeneIDi 6288040.
    KEGGi rpi:Rpic_0653.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi HSTLEMA.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci RPIC402626:GH94-654-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 1 of Ralstonia pickettii 12J."
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
      , Kyrpides N., Mikhailova N., Marsh T., Richardson P.
      Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 12J.

    Entry informationi

    Entry nameiTRMB_RALPJ
    AccessioniPrimary (citable) accession number: B2U7E2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3