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Protein

Malate dehydrogenase

Gene

mdh

Organism
Ralstonia pickettii (strain 12J)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95SubstrateUniRule annotation1
Binding sitei101SubstrateUniRule annotation1
Binding sitei108NADUniRule annotation1
Binding sitei115NADUniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Binding sitei165SubstrateUniRule annotation1
Active sitei190Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 18NADUniRule annotation7
Nucleotide bindingi132 – 134NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Rpic_2153
OrganismiRalstonia pickettii (strain 12J)
Taxonomic identifieri402626 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000002566 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001916281 – 328Malate dehydrogenaseAdd BLAST328

Interactioni

Protein-protein interaction databases

STRINGi402626.Rpic_2153.

Structurei

3D structure databases

ProteinModelPortaliB2U705.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000220953.
KOiK00024.
OMAiQYPDAGQ.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

B2U705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKAPMRVAV TGAAGQIGYA LLFRIAAGEM LGKDQPVILQ LLEIPDEKAQ
60 70 80 90 100
KALKGVMMEI EDCAFPLLAG MQAHSDPMTA FKDVDVALLV GARPRGPGME
110 120 130 140 150
RADLLSANAQ IFTAQGRALN AVASRNVKVL VVGNPANTNA YIAMKSAPDL
160 170 180 190 200
PRENFTAMLR LDHNRALSQI AAKTGKPVSS IEKLFVWGNH SPTMYADYRY
210 220 230 240 250
ATIDGKSVKD MINDPVWNND VFLPTVGKRG AAIIEARGLS SAASAANAAI
260 270 280 290 300
DHVRDWVLGT NGKIVTMGIP SNGEYGIPKD VMFGFPVTTE NGKYKIVEGL
310 320
EIDEYSQGKI KITLDELEGE RAGVQHLL
Length:328
Mass (Da):35,247
Last modified:July 1, 2008 - v1
Checksum:iB37874A02F8F76B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA. Translation: ACD27287.1.
RefSeqiWP_012436029.1. NC_010682.1.

Genome annotation databases

EnsemblBacteriaiACD27287; ACD27287; Rpic_2153.
GeneIDi6286694.
KEGGirpi:Rpic_2153.
PATRICi20255118. VBIRalPic63053_3301.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001068 Genomic DNA. Translation: ACD27287.1.
RefSeqiWP_012436029.1. NC_010682.1.

3D structure databases

ProteinModelPortaliB2U705.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi402626.Rpic_2153.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD27287; ACD27287; Rpic_2153.
GeneIDi6286694.
KEGGirpi:Rpic_2153.
PATRICi20255118. VBIRalPic63053_3301.

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000220953.
KOiK00024.
OMAiQYPDAGQ.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_RALPJ
AccessioniPrimary (citable) accession number: B2U705
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.