B2TZI7 (SURE_SHIB3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'/3'-nucleotidase SurE
EC=3.1.3.5
EC=3.1.3.6

Alternative name(s):
Exopolyphosphatase
EC=3.6.1.11
Nucleoside monophosphate phosphohydrolase

Gene names

Name:	surE
Ordered Locus Names:	SbBS512_E3130

Organism

Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) [Complete proteome] [HAMAP]

Taxonomic identifier

344609 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella › Shigella boydii

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. Also hydrolyzes polyphosphate (exopolyphosphatase activity) with the preference for short-chain-length substrates (P20-25). Might be involved in the regulation of dNTP and NTP pools, and in the turnover of 3'-mononucleotides produced by numerous intracellular RNases (T1, T2, and F) during the degradation of various RNAs By similarity. HAMAP-Rule MF_00060
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP-Rule MF_00060 A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP-Rule MF_00060 (Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate. HAMAP-Rule MF_00060
Cofactor	Binds 1 divalent metal cation per subunit By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00060.
Sequence similarities	Belongs to the SurE nucleotidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-nucleotidase activity Inferred from electronic annotation. Source: HAMAP 5'-nucleotidase activity Inferred from electronic annotation. Source: HAMAP exopolyphosphatase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	5'/3'-nucleotidase SurE HAMAP-Rule MF_00060		PRO_1000092038
Sites
	Metal binding	8	1	Divalent metal cation By similarity
	Metal binding	9	1	Divalent metal cation By similarity
	Metal binding	39	1	Divalent metal cation By similarity
	Metal binding	92	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2TZI7 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 33A7CD0AEE13C3DB	FASTA	253	26,900
	10 20 30 40 50 60 MRILLSNDDG VHAPGIQTLA KALREFADVQ VVAPDRNRSG ASNSLTLESS LRTFTFENGD 70 80 90 100 110 120 IAVQMGTPTD CVYLGVNALM RPRPDIVVSG INAGPNLGDD VIYSGTVAAA MEGRHLGFPA 130 140 150 160 170 180 LAVSLDGHKH YDTAAAVTCS ILRALCKEPL RTGRILNINV PDLPLDQIKG IRVTRCGTRH 190 200 210 220 230 240 PADQVIPQQD PRGNTLYWIG PPGGKCDAGP GTDFAAVDEG YVSITPLHVD LTAHSAQDVV 250 SDWLNSVGVG TQW

References

[1]

"Complete sequence of Shigella boydii serotype 18 strain BS512." Rasko D.A., Rosovitz M., Maurelli A.T., Myers G., Seshadri R., Cer R., Jiang L., Ravel J., Sebastian Y. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 3083-94 / BS512.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001063 Genomic DNA. Translation: ACD09778.1.
RefSeq	YP_001881513.1. NC_010658.1.
3D structure databases
ProteinModelPortal	B2TZI7.
SMR	B2TZI7. Positions 1-253.
ModBase	Search...
Protein-protein interaction databases
STRING	344609.SbBS512_E3130.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD09778; ACD09778; SbBS512_E3130.
GeneID	6272033.
KEGG	sbc:SbBS512_E3130.
PATRIC	18673414. VBIShiBoy129590_3449.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0496.
HOGENOM	HOG000122500.
KO	K03787.
OMA	IASEVWI.
ProtClustDB	PRK00346.
Enzyme and pathway databases
BioCyc	SBOY344609:GI0O-4261-MONOMER.
Family and domain databases
Gene3D	3.40.1210.10. 1 hit.
HAMAP	MF_00060. SurE.
InterPro	IPR002828. SurE-like_Pase/nucleotidase. [Graphical view]
Pfam	PF01975. SurE. 1 hit. [Graphical view]
SUPFAM	SSF64167. SurE-like_Pase/nucleotidase. 1 hit.
TIGRFAMs	TIGR00087. surE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SURE_SHIB3

Accession

Primary (citable) accession number: B2TZI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program