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Protein

Thiazole synthase

Gene

thiG

Organism
Shigella boydii serotype 18 (strain CDC 3083-94 / BS512)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Schiff-base intermediate with DXPUniRule annotation1
Binding sitei156DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:SbBS512_E4481
OrganismiShigella boydii serotype 18 (strain CDC 3083-94 / BS512)
Taxonomic identifieri344609 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000001030 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001969011 – 256Thiazole synthaseAdd BLAST256

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB2TWH8
SMRiB2TWH8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni182 – 183DXP bindingUniRule annotation2
Regioni204 – 205DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.701 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

B2TWH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRIADKTFD SHLFTGTGKF ASSQLMVEAI RASGSQLVTL AMKRVDLRQH
60 70 80 90 100
NDAILEPLIA AGVTLLPNTS GAKTAEEAIF AAHLAREALG TNWLKLEIHP
110 120 130 140 150
DARWLLPDPI ETLKAAEMLV QQGFVVLPYC GADPVLCKRL EEVGCAAVMP
160 170 180 190 200
LGAPIGSNQG LETRAMLEII IQQATVPVVV DAGIGVPSHA AQALEMGADA
210 220 230 240 250
VLVNTAIAVA DDPVNMAKAF RLAVEAGLLA RQSGPGSRSH FAHATSPLTG

FLEASA
Length:256
Mass (Da):26,900
Last modified:July 1, 2008 - v1
Checksum:i85D0536CF82D4E38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001063 Genomic DNA Translation: ACD08746.1

Genome annotation databases

EnsemblBacteriaiACD08746; ACD08746; SbBS512_E4481
KEGGisbc:SbBS512_E4481

Similar proteinsi

Entry informationi

Entry nameiTHIG_SHIB3
AccessioniPrimary (citable) accession number: B2TWH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: March 28, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome