B2TW39 (ARND_SHIB3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD
EC=3.5.1.n3

Gene names

Name:	arnD
Ordered Locus Names:	SbBS512_E2632

Organism

Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) [Complete proteome] [HAMAP]

Taxonomic identifier

344609 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella › Shigella boydii

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the deformylation of 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol to 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides By similarity. HAMAP-Rule MF_01870
Catalytic activity	4-deoxy-4-formamido-beta-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + H2O = 4-amino-4-deoxy-alpha-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + formate. HAMAP-Rule MF_01870
Pathway	Glycolipid biosynthesis; 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate biosynthesis; 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate from UDP-4-deoxy-4-formamido-beta-L-arabinose and undecaprenyl phosphate: step 2/2. HAMAP-Rule MF_01870 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP-Rule MF_01870
Sequence similarities	Belongs to the polysaccharide deacetylase family. ArnD deformylase subfamily.

Ontologies

Keywords
Biological process	Antibiotic resistance Lipid A biosynthesis Lipid biosynthesis Lipid metabolism Lipopolysaccharide biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD HAMAP-Rule MF_01870		PRO_0000383542

Sequences

	Sequence		Length	Mass (Da)
	B2TW39 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 2981FBF11081C526	FASTA	296	33,142
	10 20 30 40 50 60 MTKVGLRIDV DTFRGTREGV PRLLEILSKH NIQASIFFSV GPDNMGRHLW RLVKPQFLWK 70 80 90 100 110 120 MLRSNAASLY GWDILLAGTA WPGKEIGHAN ADIIREAAKH HEVGLHAWDH HAWQARSGNW 130 140 150 160 170 180 DRQTMIDDIA RGLRTLEEII GQPVTCSAAA GWRADQKVIE AKEAFHLRYN SDCRGAMPFR 190 200 210 220 230 240 PLLESGNPGT AQIPVTLPTW DEVIGRDVKT EDFNGWLLNR ILRDKGTPVY TIHAEVEGCA 250 260 270 280 290 YQHNFVDLLK RAAQEGVTFC PLSELLSETL PLGQVVRGNI AGREGWLGCQ QIAGSR

References

[1]

"Complete sequence of Shigella boydii serotype 18 strain BS512." Rasko D.A., Rosovitz M., Maurelli A.T., Myers G., Seshadri R., Cer R., Jiang L., Ravel J., Sebastian Y. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 3083-94 / BS512.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001063 Genomic DNA. Translation: ACD09528.1.
RefSeq	YP_001881079.1. NC_010658.1.
3D structure databases
ProteinModelPortal	B2TW39.
ModBase	Search...
Protein-protein interaction databases
STRING	344609.SbBS512_E2632.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD09528; ACD09528; SbBS512_E2632.
GeneID	6271029.
KEGG	sbc:SbBS512_E2632.
PATRIC	18672311. VBIShiBoy129590_2915.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0726.
HOGENOM	HOG000261199.
KO	K13014.
OMA	HHGWQAN.
ProtClustDB	PRK15394.
Enzyme and pathway databases
BioCyc	SBOY344609:GI0O-3980-MONOMER.
UniPathway	UPA00030. UPA00036; UER00496.
Family and domain databases
Gene3D	3.20.20.370. 2 hits.
HAMAP	MF_01870. ArnD.
InterPro	IPR023557. ArnD. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR002509. Polysac_deacetylase. [Graphical view]
Pfam	PF01522. Polysacc_deac_1. 1 hit. [Graphical view]
SUPFAM	SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARND_SHIB3

Accession

Primary (citable) accession number: B2TW39

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program