Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B2TW38

- ARNA_SHIB3

UniProt

B2TW38 - ARNA_SHIB3

Protein

Putative bifunctional polymyxin resistance protein ArnA

Gene

arnA

Organism
Shigella boydii serotype 18 (strain CDC 3083-94 / BS512)
Status
Reviewed - Annotation score: 4 out of 5- Protein uncertaini
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto-arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido-arabinose (UDP-L-Ara4FN). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides By similarity.By similarity

    Catalytic activityi

    UDP-alpha-D-glucuronate + NAD+ = UDP-beta-L-threo-pentapyranos-4-ulose + CO2 + NADH.
    10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinose = 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinose.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei6 – 61Raises pKa of active site HisBy similarity
    Binding sitei213 – 2131NADBy similarity
    Binding sitei259 – 2591UDP-glucuronate; via carbonyl oxygenBy similarity
    Binding sitei264 – 2641UDP-glucuronateBy similarity
    Active sitei300 – 3001Proton acceptor; for decarboxylase activityBy similarity
    Binding sitei326 – 3261UDP-glucuronateBy similarity
    Binding sitei358 – 3581UDP-glucuronateBy similarity
    Binding sitei479 – 4791UDP-glucuronateBy similarity
    Active sitei485 – 4851Proton donor; for decarboxylase activityBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi234 – 2352NAD bindingBy similarity

    GO - Molecular functioni

    1. coenzyme binding Source: InterPro
    2. hydroxymethyl-, formyl- and related transferase activity Source: InterPro
    3. oxidoreductase activity Source: UniProtKB-KW

    GO - Biological processi

    1. lipid A biosynthetic process Source: UniProtKB-KW
    2. lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
    3. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase, Transferase

    Keywords - Biological processi

    Antibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciSBOY344609:GI0O-2630-MONOMER.
    UniPathwayiUPA00030.
    UPA00032; UER00492.
    UPA00032; UER00494.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative bifunctional polymyxin resistance protein ArnA
    Including the following 2 domains:
    UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC:2.1.2.13)
    Alternative name(s):
    ArnAFT
    UDP-L-Ara4N formyltransferase
    UDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acid decarboxylating (EC:1.1.1.305)
    Alternative name(s):
    ArnADH
    UDP-GlcUA decarboxylase
    UDP-glucuronic acid dehydrogenase
    Gene namesi
    Name:arnA
    Ordered Locus Names:SbBS512_E2631
    OrganismiShigella boydii serotype 18 (strain CDC 3083-94 / BS512)
    Taxonomic identifieri344609 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000001030: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 526526Putative bifunctional polymyxin resistance protein ArnAPRO_0000379991Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer, formed by a dimer of trimers.By similarity

    Protein-protein interaction databases

    STRINGi344609.SbBS512_E2631.

    Structurei

    3D structure databases

    ProteinModelPortaliB2TW38.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 170170Formyltransferase ArnAFTAdd
    BLAST
    Regioni2 – 6510-formyltetrahydrofolate bindingBy similarity
    Regioni180 – 526347Dehydrogenase ArnADHAdd
    BLAST
    Regioni298 – 2992UDP-glucuronate bindingBy similarity
    Regioni392 – 40110UDP-glucuronate bindingBy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the Fmt family. UDP-L-Ara4N formyltransferase subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0451.
    HOGENOMiHOG000247761.
    KOiK10011.
    OMAiVRYCVKY.
    OrthoDBiEOG6B09WV.

    Family and domain databases

    Gene3Di3.10.25.10. 1 hit.
    3.40.50.170. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR001509. Epimerase_deHydtase.
    IPR005793. Formyl_trans_C.
    IPR002376. Formyl_transf_N.
    IPR011034. Formyl_transferase_C-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01370. Epimerase. 1 hit.
    PF02911. Formyl_trans_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50486. SSF50486. 1 hit.
    SSF53328. SSF53328. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B2TW38-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKRADAGAI VAQLRVAIAP DDIAITLHHK LCHAARQLLE QTLPAIKHGN    50
    ILEIAQRENE ATCFGRRTPD DSFLEWHKPA SVLHNMVRAV ADPWPSAFSY 100
    VGNQKFTVWS SRVHPHASKA QPGSVISIAP LLIACGDGAL EIVTGQAGDG 150
    ITMQGSQLAQ TLGLVQGSRL NSQPACTARR RTRVLILGVN GFIGNHLTER 200
    LLREDHYEVY GLDIGSDAIS RFLNHPHFHF VEGDISIHSE WIEYHVKKCD 250
    VVLPLVAIAT PIEYTRNPLR VFELDFEENL RIIRYCVKYR KRIIFPSTSE 300
    VYGMCSDKYF DEDHSNLIVG PVNKPRWIYS VSKQLLDRVI WAYGEKEGLQ 350
    FTLFRPFNWM GPRLDNLNAA RIGSSRAITQ LILNLVEGSP IKLIDGGKQK 400
    RCFTDIRDGI EALYRIIENA GNRCDGEIIN IGNPENEASI EELGKMLLAS 450
    FEKHPLRHHF PPFAGFRVVE SSSYYGKGYQ DVEHRKPSIR NAHRCLDWEP 500
    KIDMQETIDE TLDFFLRTVD LTDKPS 526
    Length:526
    Mass (Da):59,435
    Last modified:July 1, 2008 - v1
    Checksum:iAF2E061948764B2A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001063 Genomic DNA. Translation: ACD07332.1.
    RefSeqiYP_001881078.1. NC_010658.1.

    Genome annotation databases

    EnsemblBacteriaiACD07332; ACD07332; SbBS512_E2631.
    GeneIDi6270240.
    KEGGisbc:SbBS512_E2631.
    PATRICi18672309. VBIShiBoy129590_2914.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001063 Genomic DNA. Translation: ACD07332.1 .
    RefSeqi YP_001881078.1. NC_010658.1.

    3D structure databases

    ProteinModelPortali B2TW38.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 344609.SbBS512_E2631.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACD07332 ; ACD07332 ; SbBS512_E2631 .
    GeneIDi 6270240.
    KEGGi sbc:SbBS512_E2631.
    PATRICi 18672309. VBIShiBoy129590_2914.

    Phylogenomic databases

    eggNOGi COG0451.
    HOGENOMi HOG000247761.
    KOi K10011.
    OMAi VRYCVKY.
    OrthoDBi EOG6B09WV.

    Enzyme and pathway databases

    UniPathwayi UPA00030 .
    UPA00032 ; UER00492 .
    UPA00032 ; UER00494 .
    BioCyci SBOY344609:GI0O-2630-MONOMER.

    Family and domain databases

    Gene3Di 3.10.25.10. 1 hit.
    3.40.50.170. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR001509. Epimerase_deHydtase.
    IPR005793. Formyl_trans_C.
    IPR002376. Formyl_transf_N.
    IPR011034. Formyl_transferase_C-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01370. Epimerase. 1 hit.
    PF02911. Formyl_trans_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50486. SSF50486. 1 hit.
    SSF53328. SSF53328. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Shigella boydii serotype 18 strain BS512."
      Rasko D.A., Rosovitz M., Maurelli A.T., Myers G., Seshadri R., Cer R., Jiang L., Ravel J., Sebastian Y.
      Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CDC 3083-94 / BS512.

    Entry informationi

    Entry nameiARNA_SHIB3
    AccessioniPrimary (citable) accession number: B2TW38
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Could be the product of a pseudogene. The N-terminal region is truncated when compared to orthologs.Curated

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3