B2TRM3 (PPNK_CLOBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	CLL_A2399

Organism

Clostridium botulinum (strain Eklund 17B / Type B) [Complete proteome] [HAMAP]

Taxonomic identifier

508765 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP MF_00361
Cofactor	Divalent metal ions By similarity. HAMAP MF_00361
Subcellular location	Cytoplasm By similarity HAMAP MF_00361.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP MF_00361		PRO_1000120845

Sequences

	Sequence		Length	Mass (Da)
	B2TRM3 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 95589E0EF3D3DA30	FASTA	284	31,402
	10 20 30 40 50 60 MKNIGIAINP SKDYKNTILN MVKEKIKNIC NITDIEVYNS FDIKNLNLSS LDLLIVLGGD 70 80 90 100 110 120 GTLLGVAREL DDDFKAPILG INIGNLGVLS SIEISDLELA LKKLMTKDCK VHKRMMLNCE 130 140 150 160 170 180 VDINESIKNI KALNEVAVAR GTLSRMVKFK IFVDEKLYAI FKGDGLIVST PTGSTAYSFS 190 200 210 220 230 240 AGGPFICPDL EVISIVPICD HTKSMHPIVL KGDSTIKIIA QNGGDQIYLT IDGQRAIEMK 250 260 270 280 DNSVITVKKN PKSLKLLLFN DYDYFKVIRN KVLNNSKECD GEKN

References

[1]

"Complete sequence of Clostridium botulinum strain Eklund." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Eklund 17B / Type B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001056 Genomic DNA. Translation: ACD23190.1.
RefSeq	YP_001886588.1. NC_010674.1.
3D structure databases
ProteinModelPortal	B2TRM3.
ModBase	Search...
Protein-protein interaction databases
STRING	B2TRM3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6292933.
GenomeReviews	Gene locus CLL_A2399 in contig CP001056_GR.
KEGG	cbk:CLL_A2399.
PATRIC	19397618. VBICloBot123574_2314.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG713904.
OMA	KLGWGLP.
ProtClustDB	CLSK811135.
Family and domain databases
HAMAP	MF_00361. NAD_kinase. [Tree]
InterPro	IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017438. ATP-NAD_kinase_PpnK-typ_a/b. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. polyP/ATP_NADK_prd. [Graphical view]
Gene3D	G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit. G3DSA:3.40.50.10330. ATP-NAD_kinase_PpnK-typ_a/b. 1 hit.
KO	K00858.
PANTHER	PTHR20275. ATP_NADK. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_CLOBB

Accession

Primary (citable) accession number: B2TRM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program