Reviewed, UniProtKB/Swiss-Prot B2TQ47 (AROK_CLOBB)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	CLL_A3138

Organism

Clostridium botulinum (strain Eklund 17B / Type B) [Complete proteome] [HAMAP]

Taxonomic identifier

508765 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00109
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity. HAMAP MF_00109
Subcellular location	Cytoplasm By similarity HAMAP MF_00109.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	Shikimate kinase HAMAP MF_00109		PRO_1000117457
Regions
	Nucleotide binding	12 – 17	6	ATP By similarity
Sites
	Metal binding	16	1	Magnesium By similarity
	Binding site	34	1	Substrate By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	79	1	Substrate; via amide nitrogen By similarity
	Binding site	116	1	ATP By similarity
	Binding site	133	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2TQ47-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 2B6003A44AF3633B	FASTA	165	18,714
	10 20 30 40 50 60 MKNKIFLIGM PGCGKSTIGE IIAKELMLKF IDMDICIEEK TSKTISELFE QGEDYFRDIE 70 80 90 100 110 120 SETCKEIIKY DNVVIATGGG VVKKDINIET LKNNGLVIFI DRPVEKIISD IDVSRRPLLK 130 140 150 160 NGKERIIGLY KERYDIYKKA CHKIVVNGST IDEVVGEIKK IIINN

References

[1]	"Complete sequence of Clostridium botulinum strain Eklund." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001056 Genomic DNA. Translation: ACD24123.1.
RefSeq	YP_001887324.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6294764.
GenomeReviews	Gene locus CLL_A3138 in contig CP001056_GR.
KEGG	cbk:CLL_A3138.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335815.
OMA	DLSWENW.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_CLOBB

Accession

Primary (citable) accession number: B2TQ47

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 19, 2010
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)