Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Argininosuccinate synthase

Gene

argG

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei88Citrulline1
Binding sitei93Citrulline1
Binding sitei118ATP; via amide nitrogen1
Binding sitei120Aspartate1
Binding sitei124Aspartate1
Binding sitei124Citrulline1
Binding sitei125Aspartate1
Binding sitei128Citrulline1
Binding sitei177Citrulline1
Binding sitei186Citrulline1
Binding sitei263Citrulline1
Binding sitei275Citrulline1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATP9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:argG
Ordered Locus Names:CLL_A3114
OrganismiClostridium botulinum (strain Eklund 17B / Type B)
Taxonomic identifieri935198 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001195 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000890301 – 407Argininosuccinate synthaseAdd BLAST407

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliB2TQ23.
SMRiB2TQ23.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Phylogenomic databases

HOGENOMiHOG000230093.
KOiK01940.
OMAiGVGRIDM.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR11587. PTHR11587. 1 hit.
PfamiView protein in Pfam
PF00764. Arginosuc_synth. 1 hit.
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiView protein in PROSITE
PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

B2TQ23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKLNKVILA YSGGLDTSII IPWLKENYNC EVIAVCGNVG QKDELDGLEE
60 70 80 90 100
KAIKTGASKL YIEDLTKEFV EDYIFPTIQA GAIYEGKYLL GTSFARPLIG
110 120 130 140 150
KRLVEIAKAE GADAICHGCT GKGNDQVRFE LAVKAFDPDM KIIAPWRIWD
160 170 180 190 200
IKSREDEIAY AEARNVPIKI NHETNYSKDK NIWHLSHEGL DLEDPKNEPK
210 220 230 240 250
YDEILELSNS LEKAPNEPTY ITLTFEKGNA VALNGEKMDA VTLLDELNKI
260 270 280 290 300
GGKNAIGITD MVENRLVGMK SRGVYETPGG TILYKAHKDL EELCLDKETS
310 320 330 340 350
HYKEQISLKF ADLVYNGLWF TPLREALSEF IKKTQETVTG EIKLKLYKGN
360 370 380 390 400
IVNAGMTSPY SLYSEEYATF GEDAVYNQND SAGFITLYGL PTVVKAKMYQ

SLKKGTK
Length:407
Mass (Da):45,558
Last modified:July 1, 2008 - v1
Checksum:i2A35596CB3602E87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD22097.1.
RefSeqiWP_012422973.1. NC_018648.1.

Genome annotation databases

EnsemblBacteriaiACD22097; ACD22097; CLL_A3114.
GeneIDi19963642.
KEGGicbk:CLL_A3114.
PATRICifig|935198.13.peg.3078.

Similar proteinsi

Entry informationi

Entry nameiASSY_CLOBB
AccessioniPrimary (citable) accession number: B2TQ23
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: October 25, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families