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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48Substrate; via amide nitrogenUniRule annotation1
Binding sitei124ATPUniRule annotation1
Binding sitei175ATPUniRule annotation1
Binding sitei202Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:CLL_A3105
OrganismiClostridium botulinum (strain Eklund 17B / Type B)
Taxonomic identifieri935198 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001195 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003838421 – 278Hydroxyethylthiazole kinaseAdd BLAST278

Structurei

3D structure databases

ProteinModelPortaliB2TQ14.
SMRiB2TQ14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000114352.
KOiK00878.
OMAiSINDCAN.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiView protein in InterPro
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
PfamiView protein in Pfam
PF02110. HK. 1 hit.
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

B2TQ14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINEILDKVV RLLEEVRAKK PLVHSITNYI TATDCANVIL AVGGSPTMAD
60 70 80 90 100
YVKEVEEIAS ISSAVVLNMG VISDGMVESM ILAGKSANKN NVPVIFDPVG
110 120 130 140 150
AGVANFRNES AEKILSEVKI DIIRGNISEI KFICGLSSET KGVDASESDM
160 170 180 190 200
NMGNDKKAIV AQELAKKLNC VVAITGVDDI ISDGKRNVIL SNGHKMLANV
210 220 230 240 250
TGTGCMSSAL CGAFAGASND YFIAAICAIL TMGISGEIAY EKSKGIGMGT
260 270
FHTSLIDAIS MMNENIIKEK AKVTTINR
Length:278
Mass (Da):29,382
Last modified:July 1, 2008 - v1
Checksum:i48081706B98733C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD24375.1.
RefSeqiWP_012425131.1. NC_018648.1.

Genome annotation databases

EnsemblBacteriaiACD24375; ACD24375; CLL_A3105.
GeneIDi19964075.
KEGGicbk:CLL_A3105.
PATRICifig|935198.13.peg.3069.

Similar proteinsi

Entry informationi

Entry nameiTHIM_CLOBB
AccessioniPrimary (citable) accession number: B2TQ14
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 1, 2008
Last modified: August 30, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families