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Protein

Putative K(+)-stimulated pyrophosphate-energized sodium pump

Gene

hppA

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na+ movement across the membrane.UniRule annotation

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Requires K+ for maximal activity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei450 – 4501Determinant of potassium dependenceUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationImported

Keywords - Biological processi

Ion transport, Sodium transportUniRule annotation, Transport

Keywords - Ligandi

MagnesiumUniRule annotation, PotassiumUniRule annotation, Sodium

Enzyme and pathway databases

BioCyciCBOT508765:GJ4H-3040-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative K(+)-stimulated pyrophosphate-energized sodium pumpUniRule annotation (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Membrane-bound sodium-translocating pyrophosphataseUniRule annotation
Pyrophosphate-energized inorganic pyrophosphataseUniRule annotation
Gene namesi
Name:hppAUniRule annotation
Ordered Locus Names:CLL_A3052Imported
ORF Names:CB17B2968Imported
OrganismiClostridium botulinum (strain Eklund 17B / Type B)Imported
Taxonomic identifieri935198 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001195 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalUniRule annotationAdd
BLAST
Transmembranei54 – 7421HelicalUniRule annotationAdd
BLAST
Transmembranei77 – 9721HelicalUniRule annotationAdd
BLAST
Transmembranei126 – 14621HelicalUniRule annotationAdd
BLAST
Transmembranei153 – 17321HelicalUniRule annotationAdd
BLAST
Transmembranei221 – 24121HelicalUniRule annotationAdd
BLAST
Transmembranei247 – 26721HelicalUniRule annotationAdd
BLAST
Transmembranei280 – 30021HelicalUniRule annotationAdd
BLAST
Transmembranei303 – 32321HelicalUniRule annotationAdd
BLAST
Transmembranei365 – 38521HelicalUniRule annotationAdd
BLAST
Transmembranei392 – 41221HelicalUniRule annotationAdd
BLAST
Transmembranei450 – 47021HelicalUniRule annotationAdd
BLAST
Transmembranei487 – 50721HelicalUniRule annotationAdd
BLAST
Transmembranei555 – 57521HelicalUniRule annotationAdd
BLAST
Transmembranei577 – 59721HelicalUniRule annotationAdd
BLAST
Transmembranei650 – 67021HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membraneUniRule annotation, Membrane

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotation

Phylogenomic databases

eggNOGiCOG3808.
HOGENOMiHOG000007098.
OMAiTEYYTAM.
OrthoDBiEOG6TXQSF.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

B2TPW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLLYVSLIA GFIALIVVIF LAKDILKKSP GNEKMIEISG YIEEGAMAFL
60 70 80 90 100
RKEYSYLCVF IVVVAIAILV FLNYKTAIAF VVGALFSIIA GYIGMRIAVK
110 120 130 140 150
SNVRTAEAAK SGIKEALSVA FSGGTVMGLC VVGLGIIGLS IFSIVFDLNV
160 170 180 190 200
EYITGFGLGA SSIALFARVG GGIYTKAADV GADLVGKVEA GIPEDDPRNP
210 220 230 240 250
AVIADNVGDN VGDVAGMGAD LFESYVGSII SAITLGAVLV SSWGKEIVIF
260 270 280 290 300
PLVLSSIGIL ASLIGIVFVK SYKGDNPQKA LNLGSTISGA IVLVAGFIAC
310 320 330 340 350
KYLLGSYKIF LPVIAGLLIG LLIGKITEFY TSADYKSVKF IANESETGPA
360 370 380 390 400
TNIIAGLSVG MRSTVVPILL IAVGIIVSFF AIGGAKDTAL GLYGIALSAV
410 420 430 440 450
GMLSTTAITV AVDAYGPIAD NAGGIAEMCD LDDSIREITD KLDSVGNTTA
460 470 480 490 500
AIGKGFAIGS AALTALALFA SYSQIVNLET INLLNPLTLV GVLIGGMLPF
510 520 530 540 550
LFAALTMQSV GKAATQMVEE VRRQFKENDG ILKGTQKPDY SKCVEISTNA
560 570 580 590 600
ALKEMILPGI LAIAVPLLVG ILLGTEALAG VIGGGVVTGV MLAIMMANAG
610 620 630 640 650
GAWDNAKKYI ESGVHGGKGS YAHKAGVVGD TVGDPFKDTS GPSMNILIKL
660 670
MTIVSVVFAP VILKYGGILI NLFIK
Length:675
Mass (Da):69,724
Last modified:July 1, 2008 - v1
Checksum:iD98A300F27682602
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD24512.1.
FR745875 Genomic DNA. Translation: CDH91957.1.
RefSeqiWP_012425260.1. NC_010674.1.
YP_001887238.1. NC_010674.1.

Genome annotation databases

EnsemblBacteriaiACD24512; ACD24512; CLL_A3052.
GeneIDi19965447.
PATRICi19398918. VBICloBot123574_2961.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD24512.1.
FR745875 Genomic DNA. Translation: CDH91957.1.
RefSeqiWP_012425260.1. NC_010674.1.
YP_001887238.1. NC_010674.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD24512; ACD24512; CLL_A3052.
GeneIDi19965447.
PATRICi19398918. VBICloBot123574_2961.

Phylogenomic databases

eggNOGiCOG3808.
HOGENOMiHOG000007098.
OMAiTEYYTAM.
OrthoDBiEOG6TXQSF.

Enzyme and pathway databases

BioCyciCBOT508765:GJ4H-3040-MONOMER.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of Clostridium botulinum strain Eklund."
    Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Eklund 17B / Type BImported.
  2. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Eklund 17BImported.
  3. Shrivastava S., Brinkac L.M., Dodson R.J., Harkins D.M., Durkin A.S., Sutton G.
    Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Eklund 17BImported.
  4. "Genomic and physiological variability within Group II (non-proteolytic) Clostridium botulinum."
    Stringer S.C., Carter A.T., Webb M.D., Wachnicka E., Crossman L.C., Sebaihia M., Peck M.W.
    BMC Genomics 14:333-333(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: B str. Eklund 17BImported.

Entry informationi

Entry nameiB2TPW1_CLOBB
AccessioniPrimary (citable) accession number: B2TPW1
Secondary accession number(s): U4PC04
Entry historyi
Integrated into UniProtKB/TrEMBL: July 1, 2008
Last sequence update: July 1, 2008
Last modified: June 24, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.