B2TPD4 (GSA_CLOBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	hemL
Ordered Locus Names:	CLL_A2904

Organism

Clostridium botulinum (strain Eklund 17B / Type B) [Complete proteome] [HAMAP]

Taxonomic identifier

508765 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium botulinum › Clostridium botulinum B

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Glutamate-1-semialdehyde 2,1-aminomutase HAMAP-Rule MF_00375		PRO_1000121871
Amino acid modifications
	Modified residue	264	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2TPD4 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 6455DF7FA23DC18E	FASTA	427	47,203
	10 20 30 40 50 60 MNNLEIFEES KKYMPGGVNS PVRCFKEMGM NPPVIKSGKG VIIKDEDGKE YIDFVLAWGP 70 80 90 100 110 120 LLLGHCDEDV VKAIKETSEN ALAFGAPTKL ELELSKFMCE NLDNVDMIRM VNSGTEATMS 130 140 150 160 170 180 AVKLARGYTG KSKIVKFAGC YHGHFDGFLI EAGSGVLTEG IPGSLGVPKE SVENTLIGLY 190 200 210 220 230 240 NDKTQIKELF KKQGNEIAAV IIEPVAGNMG VIKANEDFIK ELRSLCDEYG ALLIFDEVMT 250 260 270 280 290 300 GFRVAFKGAQ TLFDIKPDLI TYAKIMGGGL PCGAYAGKKE IMEKLSPCGG VYQAGTMSGN 310 320 330 340 350 360 PVVMSAGLAT LTKLKNNIEL YDHVEKIGEK LQEGLIKISK ENDVPLIINR VGGMLTLFFT 370 380 390 400 410 420 ELEKVNTYED VKTCDNERFK RYFKHMLNEG FNIAPSQFEA MFLSVKHTEE HIDKFLDAFK RFAINEK

References

[1]

"Complete sequence of Clostridium botulinum strain Eklund." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Eklund 17B / Type B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001056 Genomic DNA. Translation: ACD21981.1.
RefSeq	YP_001887091.1. NC_010674.1.
3D structure databases
ProteinModelPortal	B2TPD4.
ModBase	Search...
Protein-protein interaction databases
STRING	508765.CLL_A2904.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD21981; ACD21981; CLL_A2904.
GeneID	6294866.
KEGG	cbk:CLL_A2904.
PATRIC	19398622. VBICloBot123574_2814.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0001.
HOGENOM	HOG000020210.
KO	K01845.
OMA	NANIPFF.
ProtClustDB	PRK00062.
Enzyme and pathway databases
BioCyc	CBOT508765:GJ4H-2893-MONOMER.
UniPathway	UPA00251; UER00317.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
HAMAP	MF_00375. HemL_aminotrans_3.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_CLOBB

Accession

Primary (citable) accession number: B2TPD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program