B2TPC7 (KHSE_CLOBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	CLL_A2897

Organism

Clostridium botulinum (strain Eklund 17B / Type B) [Complete proteome] [HAMAP]

Taxonomic identifier

508765 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Homoserine kinase HAMAP MF_00384		PRO_1000122411
Regions
	Nucleotide binding	83 – 93	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B2TPC7 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 071931527A34D971	FASTA	298	32,809
	10 20 30 40 50 60 MIKVRVPATS ANMGPGFDSM GMAVKVYNEF AFREINNGLN FNGVPEEFCN EDNIIYKAMK 70 80 90 100 110 120 YCFDKADYKI KGLNISVLNQ DIPISRGLGS SSSCIVGGLV GANEILGGKF SKDELLEMAV 130 140 150 160 170 180 KIEGHPDNVA PALFGGMVVA IIENNKTVYN KIEIKDKVKF ITIVPDFRLS TEKARTVLPK 190 200 210 220 230 240 QISRADGIYN IGRAALMISC FVTDRYDLIR SACNDALHQN YRKELIPHFD DVYNKCYELG 250 260 270 280 290 ALGCYLSGAG PTIMAIIDNG ANGFSNNIKQ YLKDKDIKWG VLELQADNKG AFVIKGDS

References

[1]

"Complete sequence of Clostridium botulinum strain Eklund." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Eklund 17B / Type B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001056 Genomic DNA. Translation: ACD24465.1.
RefSeq	YP_001887084.1. NC_010674.1.
3D structure databases
ProteinModelPortal	B2TPC7.
ModBase	Search...
Protein-protein interaction databases
STRING	B2TPC7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6293072.
GenomeReviews	Gene locus CLL_A2897 in contig CP001056_GR.
KEGG	cbk:CLL_A2897.
PATRIC	19398608. VBICloBot123574_2807.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646290.
OMA	NNALMST.
ProtClustDB	PRK01212.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_CLOBB

Accession

Primary (citable) accession number: B2TPC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program