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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43S-adenosyl-L-methionineUniRule annotation1
Binding sitei68S-adenosyl-L-methionineUniRule annotation1
Binding sitei101S-adenosyl-L-methionineUniRule annotation1
Binding sitei124S-adenosyl-L-methionineUniRule annotation1
Binding sitei128SubstrateUniRule annotation1
Binding sitei160SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:CLL_A0894
OrganismiClostridium botulinum (strain Eklund 17B / Type B)
Taxonomic identifieri935198 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001195 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001363451 – 219tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST219

Structurei

3D structure databases

SMRiB2TM00.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000251689.
KOiK03439.
OMAiWITFPDP.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

B2TM00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMRKKPWAR PELESCNFFI VNPKENKGKW NESFNNENPI YLELGCGKGV
60 70 80 90 100
FVAVHGSDNE NINYIAIDIK DEVLGLAKRN IEKAYNEKNK ELNNIKLMAQ
110 120 130 140 150
EIGLINEILD ENDKISRIYI NFCNPWPKKK HKKRRLTHTR QLIQYRNFLK
160 170 180 190 200
ENGEIWFKTD DDELFEESLE YFKEGKFRIE YITYDLHTSG FEGNIQTEHE
210
RMFTEQGIKT KFLIAIKED
Length:219
Mass (Da):26,104
Last modified:July 1, 2008 - v1
Checksum:i469D674AEA264656
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD24441.1.
RefSeqiWP_012425194.1. NC_018648.1.

Genome annotation databases

EnsemblBacteriaiACD24441; ACD24441; CLL_A0894.
GeneIDi19964962.
KEGGicbk:CLL_A0894.
PATRICifig|935198.13.peg.844.

Similar proteinsi

Entry informationi

Entry nameiTRMB_CLOBB
AccessioniPrimary (citable) accession number: B2TM00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: August 30, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families