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B2TM00 (TRMB_CLOBB) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:CLL_A0894
OrganismClostridium botulinum (strain Eklund 17B / Type B) [Complete proteome] [HAMAP]
Taxonomic identifier508765 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000136345

Sites

Binding site431S-adenosyl-L-methionine By similarity
Binding site681S-adenosyl-L-methionine By similarity
Binding site1011S-adenosyl-L-methionine By similarity
Binding site1241S-adenosyl-L-methionine By similarity
Binding site1281Substrate By similarity
Binding site1601Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B2TM00 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 469D674AEA264656

FASTA21926,104
        10         20         30         40         50         60 
MRMRKKPWAR PELESCNFFI VNPKENKGKW NESFNNENPI YLELGCGKGV FVAVHGSDNE 

        70         80         90        100        110        120 
NINYIAIDIK DEVLGLAKRN IEKAYNEKNK ELNNIKLMAQ EIGLINEILD ENDKISRIYI 

       130        140        150        160        170        180 
NFCNPWPKKK HKKRRLTHTR QLIQYRNFLK ENGEIWFKTD DDELFEESLE YFKEGKFRIE 

       190        200        210 
YITYDLHTSG FEGNIQTEHE RMFTEQGIKT KFLIAIKED 

« Hide

References

[1]"Complete sequence of Clostridium botulinum strain Eklund."
Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Eklund 17B / Type B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001056 Genomic DNA. Translation: ACD24441.1.
RefSeqYP_001885095.1. NC_010674.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING508765.CLL_A0894.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD24441; ACD24441; CLL_A0894.
GeneID6292011.
KEGGcbk:CLL_A0894.
PATRIC19394635. VBICloBot123574_0829.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMADNVMTEY.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycCBOT508765:GJ4H-888-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CLOBB
AccessionPrimary (citable) accession number: B2TM00
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: June 11, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways