Reviewed, UniProtKB/Swiss-Prot B2TL74 (BIOD_CLOBB)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	CLL_A0722

Organism

Clostridium botulinum (strain Eklund 17B / Type B) [Complete proteome] [HAMAP]

Taxonomic identifier

508765 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Dethiobiotin synthetase HAMAP MF_00336		PRO_1000119866
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2TL74-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: E6999A697BFFB24E	FASTA	227	25,502
	10 20 30 40 50 60 MAKGVFITAT NTDIGKTYIT ALIVKKLREA NINCGYYKAA LSGAEIINNN IVAGDAKYVY 70 80 90 100 110 120 NVANIKGNPN ECVSYIFKQA VSPHLAAKLN NVHISMDKIV ADFNYRCNEH DYITVEGSGG 130 140 150 160 170 180 IICPIYYDKE KIMLTDIIKK LKIPIILVSP SGLGSINGTI LTLEYIKKHN LVVNSIILNN 190 200 210 220 YDKSNIIHID NKRILEEMTN LPVYTCEQYS EDIDIPLKEL KQFYDFI

References

[1]	"Complete sequence of Clostridium botulinum strain Eklund." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001056 Genomic DNA. Translation: ACD22651.1.
RefSeq	YP_001884923.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6293192.
GenomeReviews	Gene locus CLL_A0722 in contig CP001056_GR.
KEGG	cbk:CLL_A0722.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KGVFITA.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CLOBB

Accession

Primary (citable) accession number: B2TL74

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)