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Protein

Alanine racemase

Gene

alr

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotationSAAS annotation

Pathway:iD-alanine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-alanine from L-alanine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine racemase (alr)
This subpathway is part of the pathway D-alanine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-alanine from L-alanine, the pathway D-alanine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei38 – 381Proton acceptor; specific for D-alanineUniRule annotation
Binding sitei136 – 1361SubstrateUniRule annotation
Active sitei267 – 2671Proton acceptor; specific for L-alanineUniRule annotation
Binding sitei315 – 3151Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciCBOT508765:GJ4H-1564-MONOMER.
UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
Gene namesi
Name:alrImported
Ordered Locus Names:CLL_A1573Imported
ORF Names:CB17B1496Imported
OrganismiClostridium botulinum (strain Eklund 17B / Type B)Imported
Taxonomic identifieri935198 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001195 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei38 – 381N6-(pyridoxal phosphate)lysineUniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
KOiK01775.
OMAiNTIPYET.
OrthoDBiEOG6PP9NJ.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.

Sequencei

Sequence statusi: Complete.

B2TKD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKIMRPVWA EIDLDAIAYN MRNIKKLAQN KDVIAVVKAD CYGHGALDVV
60 70 80 90 100
PTLLENGASR LAVAVLTEAI ELRNNNITAP IMILGYTPEY LFEEVVNYDI
110 120 130 140 150
EQTVYDLEYA KKLSHLAIKF NKKAKVHIAI DTGMGRIGFI PNEKAIKDIK
160 170 180 190 200
KIYNLKGLDV IGIFTHFSTS DETDKEYTNE QFNKFTSFID MLSKVGVKIP
210 220 230 240 250
IKHISNSGAI IDMPKTYLDS VRAGIILYGY YPSDEINKDN IKLKPALTLK
260 270 280 290 300
ASLTRVQELD INSYISYGKT FKTERKSIIA TLPIGYADGY SRLLAPGAKV
310 320 330 340 350
IINGKFSPII GRICMDQCMI DVTDIDDIHV GDEVIILGED GNLKLTANDL
360 370 380
AKSMGTINYE ILCMLKYRIP RVYMKNGKIF TVRNYL
Length:386
Mass (Da):43,373
Last modified:July 1, 2008 - v1
Checksum:i1B36636BDEEB26D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD22809.1.
FR745875 Genomic DNA. Translation: CDH90485.1.
RefSeqiWP_012423654.1. NC_010674.1.

Genome annotation databases

EnsemblBacteriaiACD22809; ACD22809; CLL_A1573.
GeneIDi19965073.
KEGGicbk:CLL_A1573.
PATRICi19395990. VBICloBot123574_1505.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD22809.1.
FR745875 Genomic DNA. Translation: CDH90485.1.
RefSeqiWP_012423654.1. NC_010674.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD22809; ACD22809; CLL_A1573.
GeneIDi19965073.
KEGGicbk:CLL_A1573.
PATRICi19395990. VBICloBot123574_1505.

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
KOiK01775.
OMAiNTIPYET.
OrthoDBiEOG6PP9NJ.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
BioCyciCBOT508765:GJ4H-1564-MONOMER.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of Clostridium botulinum strain Eklund."
    Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Eklund 17B / Type BImported.
  2. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Eklund 17BImported.
  3. Shrivastava S., Brinkac L.M., Dodson R.J., Harkins D.M., Durkin A.S., Sutton G.
    Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Eklund 17BImported.
  4. "Genomic and physiological variability within Group II (non-proteolytic) Clostridium botulinum."
    Stringer S.C., Carter A.T., Webb M.D., Wachnicka E., Crossman L.C., Sebaihia M., Peck M.W.
    BMC Genomics 14:333-333(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: B str. Eklund 17BImported.

Entry informationi

Entry nameiB2TKD2_CLOBB
AccessioniPrimary (citable) accession number: B2TKD2
Secondary accession number(s): U4PFB0
Entry historyi
Integrated into UniProtKB/TrEMBL: July 1, 2008
Last sequence update: July 1, 2008
Last modified: July 22, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.