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Protein

Catalase-peroxidase

Gene

katG

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.
2 H2O2 = O2 + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei92Transition state stabilizer1
Active sitei96Proton acceptor1
Metal bindingi259Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide
LigandHeme, Iron, Metal-binding

Protein family/group databases

PeroxiBasei6254. CboCP01.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidase (EC:1.11.1.21)
Short name:
CP
Alternative name(s):
Peroxidase/catalase
Gene namesi
Name:katG
Ordered Locus Names:CLL_A1367
OrganismiClostridium botulinum (strain Eklund 17B / Type B)
Taxonomic identifieri935198 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001195 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003547641 – 730Catalase-peroxidaseAdd BLAST730

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki95 ↔ 218Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-244)
Cross-linki218 ↔ 244Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-95)

Post-translational modificationi

Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme.

Proteomic databases

PRIDEiB2TJE9.

Interactioni

Subunit structurei

Homodimer or homotetramer.

Structurei

3D structure databases

SMRiB2TJE9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiPEEDIYW.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiView protein in InterPro
IPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
PANTHERiPTHR30555. PTHR30555. 1 hit.
PfamiView protein in Pfam
PF00141. peroxidase. 2 hits.
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiView protein in PROSITE
PS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.

Sequencei

Sequence statusi: Complete.

B2TJE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTENKCPVTG KMGKATAGSG TTNKDWWPNQ LNLNILHQNS QLSNPMSKDF
60 70 80 90 100
NYAEEFKKLD FQALKVDLYM LMTDSQIWWP ADYGNYGPLF IRMAWHSAGT
110 120 130 140 150
YRVGDGRGGG SLGLQRFAPL NSWPDNINLD KARRLLWPIK KKYGNKISWA
160 170 180 190 200
DLLILTGNCA LESMGLKTLG FGGGRVDVWE PQEDIYWGSE KEWLGDEREK
210 220 230 240 250
GDKELENPLA AVQMGLIYVN PEGPNGNPDP LGSAHDVRET FARMAMNDEE
260 270 280 290 300
TVALIAGGHT FGKCHGAASP SYVGPAPEAA PIEEQGLGWK NTYGSGNGDD
310 320 330 340 350
TIGSGLEGAW KANPTKWTMG YLKTLFKYDW ELVKSPAGAY QWLAKNVDEE
360 370 380 390 400
DMVIDAEDST KKHRPMMTTA DLGLRYDPIY EPIARNYLKN PEKFAHDFAS
410 420 430 440 450
AWFKLTHRDM GPISRYLGPE VPKESFIWQD PIPLVKHKLI TKKDITHIKK
460 470 480 490 500
KILDSGLSIS DLVATAWASA STFRGSDKRG GANGGRIRLE PQKNWEVNEP
510 520 530 540 550
KKLNNVLNTL KQIKENFNSS HSKDKKVSLA DIIILGGCVG IEQAAKRAGY
560 570 580 590 600
NINVPFIPGR TDAIQEQTDV KSFAVLEPKE DGFRNYLKTK YVVKPEDMLI
610 620 630 640 650
DRAQLLTLTA PEMTVLIGGM RVLNCNYNKS KDGVFTNRPE CLTNDFFVNL
660 670 680 690 700
LDMNTVWKPK SEDKDRFEGF DRETGELKWT ATRVDLIFGS NSQLRAIAEV
710 720 730
YACDDNKEKF IQDFIFAWNK IMNADRFEIK
Length:730
Mass (Da):82,089
Last modified:July 1, 2008 - v1
Checksum:i0EBD9CB4530E3A38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD21810.1.

Genome annotation databases

EnsemblBacteriaiACD21810; ACD21810; CLL_A1367.
KEGGicbk:CLL_A1367.
PATRICifig|935198.13.peg.1314.

Similar proteinsi

Entry informationi

Entry nameiKATG_CLOBB
AccessioniPrimary (citable) accession number: B2TJE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: July 1, 2008
Last modified: October 25, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families