Reviewed, UniProtKB/Swiss-Prot B2TJ86 (IOLD_CLOBB)

Names and origin

Protein names

Recommended name:
    3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
      Short name=THcHDO hydrolase
    EC=3.7.1.n2

Gene names

Name:	iolD
Ordered Locus Names:	CLL_A1304

Organism

Clostridium botulinum (strain Eklund 17B / Type B) [Complete proteome] [HAMAP]

Taxonomic identifier

508765 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the cleavage of the C1-C2 bond of 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy-glucuronate (5DG) By similarity.
Catalytic activity	3,5/4-trihydroxycyclohexa-1,2-dione + H2O = 5-deoxy-glucuronic acid. HAMAP MF_01669
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 3/7. HAMAP MF_01669
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding NAD Thiamine pyrophosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase HAMAP MF_01669		PRO_0000352538
Regions
	Region	441 – 521	81	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	492	1	Magnesium By similarity
	Metal binding	519	1	Magnesium By similarity
	Binding site	65	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2TJ86-1 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 8255AADD936968B5	FASTA	643	71,510
	10 20 30 40 50 60 MKSTKMTTAQ ALVKFLDNQY VSFDGKEEKF VHGIFTIFGH GIVVGLGQAL DENPRGLKVY 70 80 90 100 110 120 QGRNEQGMAH AATAFAKQNN RRKIIACSSS IGPGAANMVT AAATATVNNI PLLLLPGDSF 130 140 150 160 170 180 ATRQPDPVLQ QIEQSYNLGI TTNDVFKPVC KYWDRVNRPE QLMSAMINAM RVLTDPAETG 190 200 210 220 230 240 AVCIALPQDV QGESFEFPEY FFKKRVHKIT RPLAVEEEFY ECLNIIKNKK KPIIICGGGV 250 260 270 280 290 300 RYSEAGDTLS KFANRFNIPI GETQAGKSSI KSSDLMNLGG IGVTGNLAAN IIAKDADLVI 310 320 330 340 350 360 GVGTRFSDFT TASKSLFENP DVEFVTINLS KFHASKLDSC KMVGDVKECL EYLYKLLEKE 370 380 390 400 410 420 NYISSYKDEI KDAKMAWKEE MKKLTNIKYE ENFDPIIKFR NKESLEEFKK LTDTTITQTS 430 440 450 460 470 480 ALGLIRECID NDAIIVGASG SLPGDLQRMW ETESLNSYHM EYGYSCMGYE IAAGFGAKLA 490 500 510 520 530 540 EPEKEVYSIL GDGSYLMLHS ELITSIQENK KVNVLLFDNC GFGCINNLQM SNGIGNLATE 550 560 570 580 590 600 FRYRNDETNK LNGKLIPIDF AKAAAGYGLK TYTAKNLEEL KNALIDAKKQ RVSTLIDIKV 610 620 630 640 LPKTMTDGYE SWWHVGLAEV SEKESVNKAF ENNKKVLKSA RKY

References

[1]	"Complete sequence of Clostridium botulinum strain Eklund." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001056 Genomic DNA. Translation: ACD24665.1.
RefSeq	YP_001885502.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6294783.
GenomeReviews	Gene locus CLL_A1304 in contig CP001056_GR.
KEGG	cbk:CLL_A1304.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B2TJ86. KYLSWWR.
Family and domain databases
HAMAP	MF_01669. [Tree]
InterPro	IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLD_CLOBB

Accession

Primary (citable) accession number: B2TJ86

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	July 1, 2008
Last modified:	June 16, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)