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B2TJ61

- PNP_CLOBB

UniProt

B2TJ61 - PNP_CLOBB

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi485 – 4851MagnesiumUniRule annotation
    Metal bindingi491 – 4911MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciCBOT508765:GJ4H-1273-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:CLL_A1279
    OrganismiClostridium botulinum (strain Eklund 17B / Type B)
    Taxonomic identifieri935198 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001195: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 704704Polyribonucleotide nucleotidyltransferasePRO_1000192471Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi508765.CLL_A1279.

    Structurei

    3D structure databases

    ProteinModelPortaliB2TJ61.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini552 – 61160KHUniRule annotationAdd
    BLAST
    Domaini621 – 68969S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiRRIITHD.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF13014. KH_3. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 1 hit.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2TJ61-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNVLSTNIA GKEMKVEFGK IGMLSNAATF MSYGDTVILT NVNASSEPRV    50
    GIDFFPLSVE YEERLYAVGK IPGGFIKREG RPSEKAILNG RAVDRTLRPL 100
    FPKGYRNDVQ VVCTVVSVEK DNLPEILAIN AASMALCLSS IPFTIPVAAV 150
    QVGLIDNNFI VNPNATEREE STLHLTVCAT KERVMMIEAG GNEIPEDIMI 200
    DAIKFGFDEC QKIINFQEEA VSKFGKEKDV PTLFTVDEEV EKDIKEFASD 250
    MIKEAMYITD KDERNAAIDA VNQKVKEEFG EKYEDKFGDI KEVLYNMQKK 300
    VVRHMLLKDK RRPDGRAFDE VRPLGCEIGL LPRTHGTGLF TRGLTQVMTV 350
    ATLGAVGDIQ ILDGIDEAQS KRYMHHYNFP GYSVGEVKPL RGPGRREIGH 400
    GALAERALEP LIPSEEEFPY TIRLVSEVLS SNGSTSQASV CGSTLALLDA 450
    GVPLKRPAAG IAMGLITSED LSEEQVLTDI QGIEDFFGDM DFKVAGTTEG 500
    ITSIQVDTKL KGFSFNVVEN AIRDARKARM TILDKINECI SSPREDVSLY 550
    APKTETIQID PDKIRSVIGA GGKVINKIIQ DTGVKIDIKE DGSVFVSSSD 600
    HAGVKEAIKI IEGLTKDVKA GEIYLGKVTK ITTFGAFVEI LPNKEGLVHI 650
    SKLDKERVNK VEDVVSVGDE ILVKVTEIDS QGRINLSRKD VLLDQENKEN 700
    KEEK 704
    Length:704
    Mass (Da):77,505
    Last modified:July 1, 2008 - v1
    Checksum:i0865545A61BE1E0A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001056 Genomic DNA. Translation: ACD23650.1.
    RefSeqiWP_012424453.1. NC_010674.1.
    YP_001885477.1. NC_010674.1.

    Genome annotation databases

    EnsemblBacteriaiACD23650; ACD23650; CLL_A1279.
    GeneIDi6292083.
    KEGGicbk:CLL_A1279.
    PATRICi19395400. VBICloBot123574_1210.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001056 Genomic DNA. Translation: ACD23650.1 .
    RefSeqi WP_012424453.1. NC_010674.1.
    YP_001885477.1. NC_010674.1.

    3D structure databases

    ProteinModelPortali B2TJ61.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 508765.CLL_A1279.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACD23650 ; ACD23650 ; CLL_A1279 .
    GeneIDi 6292083.
    KEGGi cbk:CLL_A1279.
    PATRICi 19395400. VBICloBot123574_1210.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi RRIITHD.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci CBOT508765:GJ4H-1273-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF13014. KH_3. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 1 hit.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Clostridium botulinum strain Eklund."
      Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
      Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Eklund 17B / Type B.

    Entry informationi

    Entry nameiPNP_CLOBB
    AccessioniPrimary (citable) accession number: B2TJ61
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3