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B2TJ31

- TRMD_CLOBB

UniProt

B2TJ31 - TRMD_CLOBB

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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene
trmD, CLL_A1249
Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Specifically methylates guanosine-37 in various tRNAs By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101S-adenosyl-L-methionine; via amide nitrogen By similarity

GO - Molecular functioni

  1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBOT508765:GJ4H-1243-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferase (EC:2.1.1.228)
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene namesi
Name:trmD
Ordered Locus Names:CLL_A1249
OrganismiClostridium botulinum (strain Eklund 17B / Type B)
Taxonomic identifieri508765 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001195: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238tRNA (guanine-N(1)-)-methyltransferaseUniRule annotationPRO_1000130151Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi508765.CLL_A1249.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1346S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0336.
HOGENOMiHOG000016242.
KOiK00554.
OMAiLYVDFEI.
OrthoDBiEOG6J48RZ.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

B2TJ31-1 [UniParc]FASTAAdd to Basket

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MKISILTLFP EMFSIFNHSI IGRAQENNIV ELELLNIRDN TLDKHKKVDD    50
YPYGGGAGMV MAPQPIIDTI RKAKENNKGK VIFLGPRGKT FNQKMAMDLS 100
KEENLIFLCG HYEGIDERVY KHIDMEVSLG DFILTGGEMA AIPVIDSILR 150
LIPGVLGKEE SFVDESFSED LLEYPQYTRP YEFEGEHVPE ILLSGHHENI 200
RKWRRLQSLD LTRKRRPDLY KNVILTKEDK KLLGRKNK 238
Length:238
Mass (Da):27,431
Last modified:July 1, 2008 - v1
Checksum:i5ACDDB01CEE101B9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001056 Genomic DNA. Translation: ACD23339.1.
RefSeqiYP_001885447.1. NC_010674.1.

Genome annotation databases

EnsemblBacteriaiACD23339; ACD23339; CLL_A1249.
GeneIDi6293082.
KEGGicbk:CLL_A1249.
PATRICi19395340. VBICloBot123574_1180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001056 Genomic DNA. Translation: ACD23339.1 .
RefSeqi YP_001885447.1. NC_010674.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 508765.CLL_A1249.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD23339 ; ACD23339 ; CLL_A1249 .
GeneIDi 6293082.
KEGGi cbk:CLL_A1249.
PATRICi 19395340. VBICloBot123574_1180.

Phylogenomic databases

eggNOGi COG0336.
HOGENOMi HOG000016242.
KOi K00554.
OMAi LYVDFEI.
OrthoDBi EOG6J48RZ.

Enzyme and pathway databases

BioCyci CBOT508765:GJ4H-1243-MONOMER.

Family and domain databases

Gene3Di 1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPi MF_00605. TrmD.
InterProi IPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view ]
Pfami PF01746. tRNA_m1G_MT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000386. tRNA_mtase. 1 hit.
SUPFAMi SSF75217. SSF75217. 1 hit.
TIGRFAMsi TIGR00088. trmD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Clostridium botulinum strain Eklund."
    Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Eklund 17B / Type B.

Entry informationi

Entry nameiTRMD_CLOBB
AccessioniPrimary (citable) accession number: B2TJ31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: June 11, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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