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B2TJ31

- TRMD_CLOBB

UniProt

B2TJ31 - TRMD_CLOBB

Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Specifically methylates guanosine-37 in various tRNAs.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei110 – 1101S-adenosyl-L-methionine; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCBOT508765:GJ4H-1243-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
    Alternative name(s):
    M1G-methyltransferaseUniRule annotation
    tRNA [GM37] methyltransferaseUniRule annotation
    Gene namesi
    Name:trmDUniRule annotation
    Ordered Locus Names:CLL_A1249
    OrganismiClostridium botulinum (strain Eklund 17B / Type B)
    Taxonomic identifieri935198 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001195: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 238238tRNA (guanine-N(1)-)-methyltransferasePRO_1000130151Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi508765.CLL_A1249.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni129 – 1346S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RNA methyltransferase TrmD family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0336.
    HOGENOMiHOG000016242.
    KOiK00554.
    OMAiLYVDFEI.
    OrthoDBiEOG6J48RZ.

    Family and domain databases

    Gene3Di1.10.1270.20. 1 hit.
    3.40.1280.10. 1 hit.
    HAMAPiMF_00605. TrmD.
    InterProiIPR029028. Alpha/beta_knot_MTases.
    IPR002649. tRNA_m1G_MeTrfase_bac.
    IPR023148. tRNA_m1G_MeTrfase_C.
    IPR029026. tRNA_m1G_MTases_N.
    IPR016009. tRNA_MeTrfase_TRMD/TRM10.
    [Graphical view]
    PfamiPF01746. tRNA_m1G_MT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
    SUPFAMiSSF75217. SSF75217. 1 hit.
    TIGRFAMsiTIGR00088. trmD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B2TJ31-1 [UniParc]FASTAAdd to Basket

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    MKISILTLFP EMFSIFNHSI IGRAQENNIV ELELLNIRDN TLDKHKKVDD    50
    YPYGGGAGMV MAPQPIIDTI RKAKENNKGK VIFLGPRGKT FNQKMAMDLS 100
    KEENLIFLCG HYEGIDERVY KHIDMEVSLG DFILTGGEMA AIPVIDSILR 150
    LIPGVLGKEE SFVDESFSED LLEYPQYTRP YEFEGEHVPE ILLSGHHENI 200
    RKWRRLQSLD LTRKRRPDLY KNVILTKEDK KLLGRKNK 238
    Length:238
    Mass (Da):27,431
    Last modified:July 1, 2008 - v1
    Checksum:i5ACDDB01CEE101B9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001056 Genomic DNA. Translation: ACD23339.1.
    RefSeqiYP_001885447.1. NC_010674.1.

    Genome annotation databases

    EnsemblBacteriaiACD23339; ACD23339; CLL_A1249.
    GeneIDi6293082.
    KEGGicbk:CLL_A1249.
    PATRICi19395340. VBICloBot123574_1180.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001056 Genomic DNA. Translation: ACD23339.1 .
    RefSeqi YP_001885447.1. NC_010674.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 508765.CLL_A1249.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACD23339 ; ACD23339 ; CLL_A1249 .
    GeneIDi 6293082.
    KEGGi cbk:CLL_A1249.
    PATRICi 19395340. VBICloBot123574_1180.

    Phylogenomic databases

    eggNOGi COG0336.
    HOGENOMi HOG000016242.
    KOi K00554.
    OMAi LYVDFEI.
    OrthoDBi EOG6J48RZ.

    Enzyme and pathway databases

    BioCyci CBOT508765:GJ4H-1243-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1270.20. 1 hit.
    3.40.1280.10. 1 hit.
    HAMAPi MF_00605. TrmD.
    InterProi IPR029028. Alpha/beta_knot_MTases.
    IPR002649. tRNA_m1G_MeTrfase_bac.
    IPR023148. tRNA_m1G_MeTrfase_C.
    IPR029026. tRNA_m1G_MTases_N.
    IPR016009. tRNA_MeTrfase_TRMD/TRM10.
    [Graphical view ]
    Pfami PF01746. tRNA_m1G_MT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000386. tRNA_mtase. 1 hit.
    SUPFAMi SSF75217. SSF75217. 1 hit.
    TIGRFAMsi TIGR00088. trmD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Clostridium botulinum strain Eklund."
      Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
      Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Eklund 17B / Type B.

    Entry informationi

    Entry nameiTRMD_CLOBB
    AccessioniPrimary (citable) accession number: B2TJ31
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3