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B2TJ31

- TRMD_CLOBB

UniProt

B2TJ31 - TRMD_CLOBB

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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Specifically methylates guanosine-37 in various tRNAs.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101S-adenosyl-L-methionine; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBOT508765:GJ4H-1243-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
Gene namesi
Name:trmDUniRule annotation
Ordered Locus Names:CLL_A1249
OrganismiClostridium botulinum (strain Eklund 17B / Type B)
Taxonomic identifieri935198 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001195: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238tRNA (guanine-N(1)-)-methyltransferasePRO_1000130151Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi508765.CLL_A1249.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1346S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0336.
HOGENOMiHOG000016242.
KOiK00554.
OMAiLYVDFEI.
OrthoDBiEOG6J48RZ.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

B2TJ31-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKISILTLFP EMFSIFNHSI IGRAQENNIV ELELLNIRDN TLDKHKKVDD
60 70 80 90 100
YPYGGGAGMV MAPQPIIDTI RKAKENNKGK VIFLGPRGKT FNQKMAMDLS
110 120 130 140 150
KEENLIFLCG HYEGIDERVY KHIDMEVSLG DFILTGGEMA AIPVIDSILR
160 170 180 190 200
LIPGVLGKEE SFVDESFSED LLEYPQYTRP YEFEGEHVPE ILLSGHHENI
210 220 230
RKWRRLQSLD LTRKRRPDLY KNVILTKEDK KLLGRKNK
Length:238
Mass (Da):27,431
Last modified:July 1, 2008 - v1
Checksum:i5ACDDB01CEE101B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD23339.1.
RefSeqiWP_012424158.1. NC_010674.1.
YP_001885447.1. NC_010674.1.

Genome annotation databases

EnsemblBacteriaiACD23339; ACD23339; CLL_A1249.
GeneIDi19964354.
KEGGicbk:CLL_A1249.
PATRICi19395340. VBICloBot123574_1180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD23339.1 .
RefSeqi WP_012424158.1. NC_010674.1.
YP_001885447.1. NC_010674.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 508765.CLL_A1249.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD23339 ; ACD23339 ; CLL_A1249 .
GeneIDi 19964354.
KEGGi cbk:CLL_A1249.
PATRICi 19395340. VBICloBot123574_1180.

Phylogenomic databases

eggNOGi COG0336.
HOGENOMi HOG000016242.
KOi K00554.
OMAi LYVDFEI.
OrthoDBi EOG6J48RZ.

Enzyme and pathway databases

BioCyci CBOT508765:GJ4H-1243-MONOMER.

Family and domain databases

Gene3Di 1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPi MF_00605. TrmD.
InterProi IPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view ]
Pfami PF01746. tRNA_m1G_MT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000386. tRNA_mtase. 1 hit.
SUPFAMi SSF75217. SSF75217. 1 hit.
TIGRFAMsi TIGR00088. trmD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Clostridium botulinum strain Eklund."
    Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Eklund 17B / Type B.

Entry informationi

Entry nameiTRMD_CLOBB
AccessioniPrimary (citable) accession number: B2TJ31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3