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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates guanosine-37 in various tRNAs.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
Gene namesi
Name:trmDUniRule annotation
Ordered Locus Names:CLL_A1249
OrganismiClostridium botulinum (strain Eklund 17B / Type B)
Taxonomic identifieri935198 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001195 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001301511 – 238tRNA (guanine-N(1)-)-methyltransferaseAdd BLAST238

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 134S-adenosyl-L-methionine bindingUniRule annotation6

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000016242.
KOiK00554.
OMAiNRYDLYL.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

B2TJ31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKISILTLFP EMFSIFNHSI IGRAQENNIV ELELLNIRDN TLDKHKKVDD
60 70 80 90 100
YPYGGGAGMV MAPQPIIDTI RKAKENNKGK VIFLGPRGKT FNQKMAMDLS
110 120 130 140 150
KEENLIFLCG HYEGIDERVY KHIDMEVSLG DFILTGGEMA AIPVIDSILR
160 170 180 190 200
LIPGVLGKEE SFVDESFSED LLEYPQYTRP YEFEGEHVPE ILLSGHHENI
210 220 230
RKWRRLQSLD LTRKRRPDLY KNVILTKEDK KLLGRKNK
Length:238
Mass (Da):27,431
Last modified:July 1, 2008 - v1
Checksum:i5ACDDB01CEE101B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD23339.1.
RefSeqiWP_012424158.1. NC_018648.1.

Genome annotation databases

EnsemblBacteriaiACD23339; ACD23339; CLL_A1249.
GeneIDi19964354.
KEGGicbk:CLL_A1249.
PATRICi19395340. VBICloBot123574_1180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD23339.1.
RefSeqiWP_012424158.1. NC_018648.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD23339; ACD23339; CLL_A1249.
GeneIDi19964354.
KEGGicbk:CLL_A1249.
PATRICi19395340. VBICloBot123574_1180.

Phylogenomic databases

HOGENOMiHOG000016242.
KOiK00554.
OMAiNRYDLYL.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMD_CLOBB
AccessioniPrimary (citable) accession number: B2TJ31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: November 2, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.