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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Specifically methylates guanosine-37 in various tRNAs.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
Gene namesi
Name:trmDUniRule annotation
Ordered Locus Names:CLL_A1249
OrganismiClostridium botulinum (strain Eklund 17B / Type B)
Taxonomic identifieri935198 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001195 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001301511 – 238tRNA (guanine-N(1)-)-methyltransferaseAdd BLAST238

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

SMRiB2TJ31
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 134S-adenosyl-L-methionine bindingUniRule annotation6

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000016242
KOiK00554
OMAiILCGHYK

Family and domain databases

Gene3Di1.10.1270.20, 1 hit
3.40.1280.10, 1 hit
HAMAPiMF_00605 TrmD, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR002649 tRNA_m1G_MeTrfase_bac
IPR023148 tRNA_m1G_MeTrfase_C
IPR029026 tRNA_m1G_MTases_N
IPR016009 tRNA_MeTrfase_TRMD/TRM10
PANTHERiPTHR32125:SF1 PTHR32125:SF1, 1 hit
PfamiView protein in Pfam
PF01746 tRNA_m1G_MT, 1 hit
PIRSFiPIRSF000386 tRNA_mtase, 1 hit
SUPFAMiSSF75217 SSF75217, 1 hit
TIGRFAMsiTIGR00088 trmD, 1 hit

Sequencei

Sequence statusi: Complete.

B2TJ31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKISILTLFP EMFSIFNHSI IGRAQENNIV ELELLNIRDN TLDKHKKVDD
60 70 80 90 100
YPYGGGAGMV MAPQPIIDTI RKAKENNKGK VIFLGPRGKT FNQKMAMDLS
110 120 130 140 150
KEENLIFLCG HYEGIDERVY KHIDMEVSLG DFILTGGEMA AIPVIDSILR
160 170 180 190 200
LIPGVLGKEE SFVDESFSED LLEYPQYTRP YEFEGEHVPE ILLSGHHENI
210 220 230
RKWRRLQSLD LTRKRRPDLY KNVILTKEDK KLLGRKNK
Length:238
Mass (Da):27,431
Last modified:July 1, 2008 - v1
Checksum:i5ACDDB01CEE101B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA Translation: ACD23339.1
RefSeqiWP_012424158.1, NC_018648.1

Genome annotation databases

EnsemblBacteriaiACD23339; ACD23339; CLL_A1249
GeneIDi19964354
KEGGicbk:CLL_A1249

Similar proteinsi

Entry informationi

Entry nameiTRMD_CLOBB
AccessioniPrimary (citable) accession number: B2TJ31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 1, 2008
Last modified: February 28, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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