B2TJ05 (RNPH_CLOBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	CLL_A0417

Organism

Clostridium botulinum (strain Eklund 17B / Type B) [Complete proteome] [HAMAP]

Taxonomic identifier

508765 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Ribonuclease PH HAMAP MF_00564		PRO_1000129333

Sequences

	Sequence		Length	Mass (Da)
	B2TJ05 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 6E3D53D99B295CE3	FASTA	249	27,853
	10 20 30 40 50 60 MRIDGRKNEQ IRHVKVTRHY TKYAEGSVYI EVGDTKVLCN VSIEDKVPPF MKGTGSGWIT 70 80 90 100 110 120 AEYNMLPRST ETRKIRDIAR LKIDGRTMEI QRLIGRALRS VVDLKALGEK TLWIDCDVIQ 130 140 150 160 170 180 ADGGTRTTAI SGAFIALVDA VNKLHKIKPF KIYPIRSFVA AVSVGIVNEE KILDLCYQED 190 200 210 220 230 240 SKAKVDMNII MTDEGSFVEV QGTGEESPYT RAELNELLDL GEKGIKQMIN VQKESLKMDS LWIGTGGNN

References

[1]

"Complete sequence of Clostridium botulinum strain Eklund." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Eklund 17B / Type B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001056 Genomic DNA. Translation: ACD23175.1.
RefSeq	YP_001884652.1. NC_010674.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2TJ05.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6292545.
GenomeReviews	Gene locus CLL_A0417 in contig CP001056_GR.
KEGG	cbk:CLL_A0417.
PATRIC	19393689. VBICloBot123574_0386.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG737187.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_CLOBB

Accession

Primary (citable) accession number: B2TJ05

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program