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Protein

Elongation factor Tu

Gene

tuf

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi81 – 855GTPUniRule annotation
Nucleotide bindingi136 – 1394GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-HAMAP
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factorUniRule annotation

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBOT508765:GJ4H-222-MONOMER.
CBOT508765:GJ4H-234-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotationImported
Synonyms:tufBImported
Ordered Locus Names:CLL_A0224Imported, CLL_A0236Imported
ORF Names:CB17B0192Imported
OrganismiClostridium botulinum (strain Eklund 17B / Type B)Imported
Taxonomic identifieri935198 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001195: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi508765.CLL_A0236.

Family & Domainsi

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
OMAiDEGGRHN.
OrthoDBiEOG6R5C6X.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2TIG1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKEKFERSK PHVNIGTIGH VDHGKTTLTA AITTVLANRG FAEAFNYAEI
60 70 80 90 100
DKAPEEKERG ITINTAHVEY ETENRHYAHV DCPGHADYVK NMITGAAQMD
110 120 130 140 150
GAILVCSAAD GPMPQTREHI LLASRVGVDY IVVFLNKADM VDDEELLELV
160 170 180 190 200
EMEVRELLSE YNFPGDDIPV IKGSALKALE NPTDETAIAP ILELMEAVDS
210 220 230 240 250
YIPTPERATD KPFIMPVEDV FTITGRGTVA TGRVETGILH VGDEVEIVGL
260 270 280 290 300
SEEKKKVVVT GIEMFRKLLD EAQAGDNIGA LLRGVQRADI ERGQVIAVPN
310 320 330 340 350
SVHPHTKFVG QVYVLKKEEG GRHTPFFDGY RPQFYFRTTD VTGSIKLPDG
360 370 380 390
MEMVMPGDHI DMNVELITPV AMDEGLRFAI REGGRTVGSG VVTKINA
Length:397
Mass (Da):43,569
Last modified:July 1, 2008 - v1
Checksum:i34E040177E382AB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD23275.1.
CP001056 Genomic DNA. Translation: ACD24211.1.
FR745875 Genomic DNA. Translation: CDH89184.1.
RefSeqiWP_012424099.1. NC_010674.1.
YP_001884459.1. NC_010674.1.
YP_001884471.1. NC_010674.1.

Genome annotation databases

EnsemblBacteriaiACD23275; ACD23275; CLL_A0236.
ACD24211; ACD24211; CLL_A0224.
GeneIDi19963991.
19966732.
PATRICi19393303. VBICloBot123574_0193.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD23275.1.
CP001056 Genomic DNA. Translation: ACD24211.1.
FR745875 Genomic DNA. Translation: CDH89184.1.
RefSeqiWP_012424099.1. NC_010674.1.
YP_001884459.1. NC_010674.1.
YP_001884471.1. NC_010674.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi508765.CLL_A0236.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD23275; ACD23275; CLL_A0236.
ACD24211; ACD24211; CLL_A0224.
GeneIDi19963991.
19966732.
PATRICi19393303. VBICloBot123574_0193.

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
OMAiDEGGRHN.
OrthoDBiEOG6R5C6X.

Enzyme and pathway databases

BioCyciCBOT508765:GJ4H-222-MONOMER.
CBOT508765:GJ4H-234-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of Clostridium botulinum strain Eklund."
    Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Eklund 17B / Type BImported.
  2. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Eklund 17BImported.
  3. Shrivastava S., Brinkac L.M., Dodson R.J., Harkins D.M., Durkin A.S., Sutton G.
    Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Eklund 17BImported.
  4. "Genomic and physiological variability within Group II (non-proteolytic) Clostridium botulinum."
    Stringer S.C., Carter A.T., Webb M.D., Wachnicka E., Crossman L.C., Sebaihia M., Peck M.W.
    BMC Genomics 14:333-333(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: B str. Eklund 17BImported.

Entry informationi

Entry nameiB2TIG1_CLOBB
AccessioniPrimary (citable) accession number: B2TIG1
Secondary accession number(s): U4P1I3
Entry historyi
Integrated into UniProtKB/TrEMBL: July 1, 2008
Last sequence update: July 1, 2008
Last modified: February 4, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.