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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Clostridium botulinum (strain Eklund 17B / Type B)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBOT508765:GJ4H-172-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:CLL_A0173
OrganismiClostridium botulinum (strain Eklund 17B / Type B)
Taxonomic identifieri935198 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001195 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Formate--tetrahydrofolate ligasePRO_1000146677Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi508765.CLL_A0173.

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2TI29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTDIEIAQE AEMLHIKNVA EKLGLDEENI EYYGKYKCKV SLDVYNKVKN
60 70 80 90 100
NRDGKLVLVT AINPTPAGEG KSTVTVGLGD ALNKMGKNTV IALREPSLGP
110 120 130 140 150
VFGIKGGAAG GGYAQVVPME DINLHFTGDM HAITSANNLL SAAIDNHIHQ
160 170 180 190 200
GNNLRIDSRR IIFKRVMDMN DRALRKIIVG MGGKINGFVR EDGFTITVAS
210 220 230 240 250
EIMAILCLAS DLEDLKHRMG DILIAYDLDG NPVYAKQLEI QGAMALLMKD
260 270 280 290 300
AIKPNLVQTL ENTPALIHGG PFANIAHGCN SIMATKLSLK LGDIVVTEAG
310 320 330 340 350
FGADLGAEKF FDIKCRYGNL KPCCVVIVAT IRALKHHGGV AKADLNTPNV
360 370 380 390 400
EALRLGIANL EKQIENIKKF NVEPVVAINK FVSDSDEEVE FIKEFCEKLG
410 420 430 440 450
VKVALSDVWA KGGDGGIELG EAVLDVIEKD KSNFKTLYDT DKTIEEKILT
460 470 480 490 500
IAKEIYGADG VVYSNEAKKQ IGELVKFNLD KLPICMAKTQ YSLSDNPNLL
510 520 530 540 550
AKPSGFNINV QEIRVSNGAG FIVVQTGNIM TMPGLPKVPA ANKMDVLKDG

EIVGLF
Length:556
Mass (Da):60,252
Last modified:June 30, 2008 - v1
Checksum:i93134614A9466369
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD22749.1.
RefSeqiYP_001884420.1. NC_010674.1.

Genome annotation databases

EnsemblBacteriaiACD22749; ACD22749; CLL_A0173.
PATRICi19393203. VBICloBot123574_0154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001056 Genomic DNA. Translation: ACD22749.1.
RefSeqiYP_001884420.1. NC_010674.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi508765.CLL_A0173.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD22749; ACD22749; CLL_A0173.
PATRICi19393203. VBICloBot123574_0154.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciCBOT508765:GJ4H-172-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Clostridium botulinum strain Eklund."
    Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Eklund 17B / Type B.

Entry informationi

Entry nameiFTHS_CLOBB
AccessioniPrimary (citable) accession number: B2TI29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2009
Last sequence update: June 30, 2008
Last modified: March 31, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.