B2TI01 (SYN_CLOBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	CLL_A0142

Organism

Clostridium botulinum (strain Eklund 17B / Type B) [Complete proteome] [HAMAP]

Taxonomic identifier

508765 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm By similarity HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Asparagine--tRNA ligase HAMAP MF_00534		PRO_1000128205

Sequences

	Sequence		Length	Mass (Da)
	B2TI01 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: F5595D1D0155E01A	FASTA	464	53,342
	10 20 30 40 50 60 MKNTVLIKKI YRETDQFLSK EVMISGWIRT LRASNAFGFI EINDGSFFKN IQVVFDDKLG 70 80 90 100 110 120 NFKEISKLPI SSSISVVGTL VATPDAKQPF EIQAKEIVIE GMSNSDYPLQ KKRHTFEYLR 130 140 150 160 170 180 SIAHLRPRSN AFSATFRVRS VAAFAIHKFF QEQGFVYTHT PIITGSDCEG AGEMFRVTTL 190 200 210 220 230 240 DPKAPELTKE GDIDYTKDFF GKETNLTVSG QLNAECFALA FRNIYTFGPT FRAENSNTTR 250 260 270 280 290 300 HAAEFWMIEP EIAFADLQDD MELAEAMLKY VIKYVMDECP EELQFFNSFV DKGLLERLNH 310 320 330 340 350 360 VVSSDFAKVT YTEAVEILEK CDKEFDYDVS WGIDLQTEHE RYLTEEHFKK PLFVTDYPKE 370 380 390 400 410 420 IKAFYMRMNE DNKTVAATDL LVPGIGEIIG GSQREERLDV LEARMAELGL KKEDYWWYLE 430 440 450 460 LRKYGETKHA GFGLGFERLI MYITGMTNIR DVIPFPRTPG TSEF

References

[1]

"Complete sequence of Clostridium botulinum strain Eklund." Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Eklund 17B / Type B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001056 Genomic DNA. Translation: ACD23255.1.
RefSeq	YP_001884396.1. NC_010674.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2TI01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6292907.
GenomeReviews	Gene locus CLL_A0142 in contig CP001056_GR.
KEGG	cbk:CLL_A0142.
PATRIC	19393139. VBICloBot123574_0129.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG745843.
OMA	AIHRFFH.
ProtClustDB	PRK03932.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_CLOBB

Accession

Primary (citable) accession number: B2TI01

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program