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B2THV2 (B2THV2_CLOBB) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP MF_02003
Gene names
Name:ileS HAMAP MF_02003 EMBL ACD22269.1
Ordered Locus Names:CLL_A0093
OrganismClostridium botulinum (strain Eklund 17B / Type B) [Complete proteome] [HAMAP]
Taxonomic identifier508765 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length1042 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
B2THV2 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: DBCB2AF596E8AFE0

FASTA1,042119,789
        10         20         30         40         50         60 
MKGENRMYKK VDSSKGFVNM EHDIATLWNE KDIIKKSFDS NQDGEYFTFY DGPPTANGKP 

        70         80         90        100        110        120 
HVGHILTRVM KDIIPRYKVM KGYKVIRKAG WDTHGLPVEL EIEKKLGISG KEQIEEYGVE 

       130        140        150        160        170        180 
KFVKECKESV FTYVSMWEKM TEQIGYWVDM EHPYVTYHNP YIESVWWALK QMWDKELLYE 

       190        200        210        220        230        240 
GHKVMPYCPR CGTALSSHEV AQGYKDVKDL TAIAKFKVQG EENKFILAWT TTPWTLPSNL 

       250        260        270        280        290        300 
ALCINKAYTY IEAKVEEEVY VLAKDLADKV LGEDYEIIRE FKGTELLGIK YEQLMPFGKV 

       310        320        330        340        350        360 
EGKAFEVIHG DYVTLTDGTG IVHIAPAYGE DDSLVAKANG IAFINLVDKE GKFVEEVTPW 

       370        380        390        400        410        420 
AGKFVKKCDD SICKWLEENN KLFKTARHLH SYPHCWRCDT PLLYYPKESW FVRMTSLRDK 

       430        440        450        460        470        480 
LLENNNKINW CPDNIRTGRF GKFLENVIDW GISRDRYWGT PLPIWECECG HKECIGSIAE 

       490        500        510        520        530        540 
LKEKGINVPE DIELHKPYID NVHLKCAHCG KEMKRTKEVI DCWFDSGSMP FAQLHYPFEN 

       550        560        570        580        590        600 
KELFEQNYPA QFISEAVDQT RGWFYTLLAI STAVFDRNPF ENCIVLGHVL DKKGLKMSKS 

       610        620        630        640        650        660 
KGNVVDPFEV LSSQGADATR WHFYTASAPW LPTRFSTDDV GESQRKFLST LWNVYSFYVL 

       670        680        690        700        710        720 
YAEIDSFNPL EYTDFKSENV MDKWIMSKLN TLIKTVDEKL NSYDITAAAL SIEEFTDELS 

       730        740        750        760        770        780 
NWYVRRNRSR YWTETINDDK IGAYVTLYRV LVTLSKVAAP FVPFITEEIY QNLVVNLDNT 

       790        800        810        820        830        840 
AEESIHLTSW PQVEESAINK ELEKEMDLAY TIVKLGRSAR NGANIKNRQP LSEILVSVAS 

       850        860        870        880        890        900 
LPEYYGDIIT NELNIKEIKF GADLSEHVNF ELKPNLPVLG RAYGKLIPGI RKEIAARNQM 

       910        920        930        940        950        960 
ELAQKIKGGN VEIINVDGTE IELNSESILV TMQGLEGYAF AGEGEFGVVL DTHITDELRE 

       970        980        990       1000       1010       1020 
EGHVREVISK IQNMRKDNGF EVADKIKLYV ANNELLLGII KKFSETIKKE TLTCDIIYNE 

      1030       1040 
ICDYTQTIIN GENLDMTVEV VK

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References

[1]"Complete sequence of Clostridium botulinum strain Eklund."
Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001056 Genomic DNA. Translation: ACD22269.1.
RefSeqYP_001884347.1. NC_010674.1.

3D structure databases

ProteinModelPortalB2THV2.
ModBaseSearch...

Protein-protein interaction databases

STRINGB2THV2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6295249.
GenomeReviewsGene locus CLL_A0093 in contig CP001056_GR.
KEGGcbk:CLL_A0093.
PATRIC19393045. VBICloBot123574_0082.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG577712.
OMAPYITYDN.
ProtClustDBPRK06039.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 2 hits.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB2THV2_CLOBB
AccessionPrimary (citable) accession number: B2THV2
Entry history
Integrated into UniProtKB/TrEMBL: July 1, 2008
Last sequence update: July 1, 2008
Last modified: January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info